Leishmania amazonensis: Anionic currents expressed in oocytes upon microinjection of mRNA from the parasite

Transport mechanisms involved in pH homeostasis are relevant for the survival of Leishmania parasites. The presence of chloride conductive pathways in Leishmania has been anticipated since anion channel inhibitors limit the proton extrusion mediated by the H +ATPase, which is the major regulator of...

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Bibliographic Details
Published in:Experimental parasitology 2007-06, Vol.116 (2), p.163-170
Main Authors: Lagos M, Luisa F., Moran, Oscar, Camacho, Marcela
Format: Article
Language:English
Subjects:
4,4′-diisothiocyanatostilbene-2,2′ disulphonic acid
Animals
Biological and medical sciences
Chloride current
DIDS
endogenous calcium-dependent chloride current
endogenous chloride current occurring after hyposmotic challenge
Fundamental and applied biological sciences. Psychology
Heterologous expression
Hydrogen-Ion Concentration
ICl,Ca
ICl,swell
Leishmania
Leishmania amazonensis
Leishmania mexicana - chemistry
Leishmania mexicana - genetics
Life cycle. Host-agent relationship. Pathogenesis
Macrophages - chemistry
Macrophages - physiology
membrane potential
Membrane Potentials - drug effects
Microinjections
Oocytes - physiology
Parasite
Patch-Clamp Techniques
Poly A - genetics
Protozoa
RNA, Messenger - administration & dosage
RNA, Messenger - pharmacology
RNA, Messenger - physiology
RNA, Protozoan - administration & dosage
RNA, Protozoan - pharmacology
RNA, Protozoan - physiology
Voltage-Dependent Anion Channels - drug effects
Voltage-Dependent Anion Channels - physiology
Xenopus laevis
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Summary:Transport mechanisms involved in pH homeostasis are relevant for the survival of Leishmania parasites. The presence of chloride conductive pathways in Leishmania has been anticipated since anion channel inhibitors limit the proton extrusion mediated by the H +ATPase, which is the major regulator of intracellular pH in amastigotes. In this study, we used Xenopus laevis oocytes as a heterologous expression system in which to study the expression of ion channels upon microinjection of polyA mRNA from Leishmania amazonensis. After injection of polyA mRNA into the oocytes, we measured three different types of currents. We discuss the possible origin of each, and propose that Type 3 currents could be the result of the heterologous expression of proteins from Leishmania since they show different pharmacological and biophysical properties as compared to endogenous oocyte currents.
ISSN:0014-4894
1090-2449
DOI:10.1016/j.exppara.2006.12.010