Production of glutaminase (E.C.3.2.1.5) from Zygosaccharomyces rouxii: Statistical optimization using response surface methodology

A face centered central composite design was employed to investigate the interactive effects of four variables, viz. concentrations of sucrose, yeast extract, sodium chloride, and glutamine, identified earlier by one-factor-at-a-time approach, on glutaminase production by Zygosaccharomyces rouxii. A...

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Bibliographic Details
Published in:	Bioresource technology 2008-07, Vol.99 (10), p.4300-4307
Main Authors:	Iyer, Padma, Singhal, Rekha S.
Format:	Article
Language:	eng
Subjects:	Analysis of Variance Biological and medical sciences Biomass Bioreactors Biotechnology - methods Culture Media Fermentation Fundamental and applied biological sciences. Psychology Glutaminase Glutaminase - analysis Glutaminase - chemistry Glutamine - analysis Models, Chemical Models, Statistical Response surface methodology Sodium Chloride - analysis Specific activity Sucrose - analysis Sucrose - chemistry Surface Properties Time Factors Zygosaccharomyces - enzymology Zygosaccharomyces rouxii
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Summary:	A face centered central composite design was employed to investigate the interactive effects of four variables, viz. concentrations of sucrose, yeast extract, sodium chloride, and glutamine, identified earlier by one-factor-at-a-time approach, on glutaminase production by Zygosaccharomyces rouxii. A significant influence of yeast extract on glutaminase production was noted. Response surface methodology (RSM) showed that a medium containing (g/l) sucrose, 17.8; yeast extract, 48.0; glutamine, 5.0 and sodium chloride, 55.6 to be optimum for the production of glutaminase. This medium was projected to produce, theoretically, an enzyme activity of 149.98 U/l and a specific activity of 0.488 U/mg protein. The applied methodology was validated using this optimized media and enzyme activity 155.89 ± 1.68 U/l and specific activity of 0.468 ± 0.088 U/mg protein was obtained. Further, this optimization strategy combined with an increase in inoculum enhanced the enzyme activity and specific activity by 2.94 and 3.58 fold, respectively, as compared to the unoptimized media.
ISSN:	0960-8524 1873-2976