Selective and rapid liquid chromatography/negative-ion electrospray ionization mass spectrometry method for the quantification of valacyclovir and its metabolite in human plasma

A rapid, sensitive and specific method was developed for the quantification of valacyclovir and acyclovir in human plasma. Sample preparation was performed by protein precipitation with acetonitrile followed by filtration. Valacyclovir, acyclovir and ganciclovir (internal standard) were separated is...

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Bibliographic Details
Published in:	Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2008-03, Vol.864 (1), p.78-86
Main Authors:	Kasiari, Maria, Gikas, Evagelos, Georgakakou, Sofia, Kazanis, Michael, Panderi, Irene
Format:	Article
Language:	eng
Subjects:	Acyclovir Acyclovir - analogs & derivatives Acyclovir - blood Analysis Analytical, structural and metabolic biochemistry Antiviral Agents - blood Biological and medical sciences Chromatography, High Pressure Liquid - methods Female Fundamental and applied biological sciences. Psychology Ganciclovir General pharmacology Human plasma Humans LC–ESI/MS Male Medical sciences Pharmacology. Drug treatments Quality Control Reproducibility of Results Sensitivity and Specificity Spectrometry, Mass, Electrospray Ionization - methods Valacyclovir Valine - analogs & derivatives Valine - blood
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Summary:	A rapid, sensitive and specific method was developed for the quantification of valacyclovir and acyclovir in human plasma. Sample preparation was performed by protein precipitation with acetonitrile followed by filtration. Valacyclovir, acyclovir and ganciclovir (internal standard) were separated isocratically on a reversed-phase porous graphitized carbon analytical column (2.1 mm × 125.0 mm i.d., particle size 5 μm), using a mobile phase of acetonitrile/water with 0.05% (v/v) diethylamine (50:50, v/v) at a flow rate of 0.15 mL min −1 in 4.0 min. Detection was performed by negative electrospray ionization using the selected ion monitoring mode of the deprotonated molecular ions at m/ z 323.0 for valacyclovir, 224.0 for acyclovir and 254.0 for ganciclovir. The assay had linear calibration curves over the range 0.020–0.800 μg mL −1 for valacyclovir and 0.100–20.00 μg mL −1 for acyclovir. Accuracy and precision were within the acceptance limit of 15%. The method was successfully applied to the analysis of plasma samples obtained from patients after oral administration of valacyclovir.
ISSN:	1570-0232 1873-376X