Sex Identification of Owls (Family Strigidae) Using Oligonucleotide Microarrays

Molecular sexing of the diversified avian family Strigidae is difficult. Sex identification using the intron length difference between W and Z chromosomal CHD1 genes, as visualized by agarose gel electrophoreses, often produces ambiguous results. Here we describe a simple method for sexing a variety...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of heredity 2008-03, Vol.99 (2), p.187-192
Main Authors: Wang, Lih Chiann, Severinghaus, Lucia Liu, Chen, Chi Tsong, Liu, Lu Yuan, Pan, Chu Hsiang, Huang, Dean, Lee, Hsiao Yuan, Lir, Jihn Tsair, Chin, Shih Chien, Pu, Chang En, Wang, Ching Ho
Format: Article
Language:English
Subjects:
Animals
Deoxyribonucleic acid
DNA
Female
Gender
Heterozygote
Male
Oligonucleotide Array Sequence Analysis - methods
Owls
Scientific method
Strigidae
Strigiformes - genetics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Molecular sexing of the diversified avian family Strigidae is difficult. Sex identification using the intron length difference between W and Z chromosomal CHD1 genes, as visualized by agarose gel electrophoreses, often produces ambiguous results. Here we describe a simple method for sexing a variety of Strigidae species using oligonucleotide microarrays, on which several sex-specific probes operated complementarily or in concert. The sex of 8 owl species was identified clearly on the microarrays through sequence recognition. This sequence-directed method can be easily applied to a wider range of Strigidae species.
ISSN:0022-1503
1465-7333
1471-8505
DOI:10.1093/jhered/esm107