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Cellular Localization of Placenta-Specific Human Endogenous Retrovirus (HERV) Transcripts and their Possible Implication in Pregnancy-Induced Hypertension
Abstract We previously investigated tissue specificity and temporal changes in expression of five human endogenous retroviruses (HERVs), including syncytin/ERVWE1 and syncytin 2 . In the current study, we examined the cellular localization and quantified the transcripts of five HERVs, syncytin , syn...
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| Published in: | Placenta (Eastbourne) 2008-03, Vol.29 (3), p.282-289 |
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| container_title | Placenta (Eastbourne) |
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| creator | Kudaka, W Oda, T Jinno, Y Yoshimi, N Aoki, Y |
| description | Abstract We previously investigated tissue specificity and temporal changes in expression of five human endogenous retroviruses (HERVs), including syncytin/ERVWE1 and syncytin 2 . In the current study, we examined the cellular localization and quantified the transcripts of five HERVs, syncytin , syncytin 2 , HERV-H7/F(XA34), HERV-Fb1, and HERV-HML6-c14, in order to elucidate their physiological and etiological roles in the placenta and in pregnancy-induced hypertension (PIH), respectively. In situ hybridization revealed trophoblast-specific transcription of all five HERVs. Consistent with a previous immunohistochemical analysis, syncytin 2 transcripts were detected only in cytotrophoblasts. All the HERVs except HERV-HML6-c14 (HML6-c14) were predominantly localized in the cytoplasm of syncytiotrophoblasts and/or cytotrophoblasts. Quantitative analysis showed that transcriptional levels of these four HERVs were lower in placentas obtained from pregnant women with PIH ( n = 22) than in those from normotensive pregnant women ( n = 87) and that the differences were statistically significant ( p = 0.001, 0.01, |
| doi_str_mv | 10.1016/j.placenta.2007.11.009 |
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In the current study, we examined the cellular localization and quantified the transcripts of five HERVs, syncytin , syncytin 2 , HERV-H7/F(XA34), HERV-Fb1, and HERV-HML6-c14, in order to elucidate their physiological and etiological roles in the placenta and in pregnancy-induced hypertension (PIH), respectively. In situ hybridization revealed trophoblast-specific transcription of all five HERVs. Consistent with a previous immunohistochemical analysis, syncytin 2 transcripts were detected only in cytotrophoblasts. All the HERVs except HERV-HML6-c14 (HML6-c14) were predominantly localized in the cytoplasm of syncytiotrophoblasts and/or cytotrophoblasts. Quantitative analysis showed that transcriptional levels of these four HERVs were lower in placentas obtained from pregnant women with PIH ( n = 22) than in those from normotensive pregnant women ( n = 87) and that the differences were statistically significant ( p = 0.001, 0.01, <0.001, and 0.04 for syncytin , syncytin 2 , HERV-H7/F(XA34), and HERV-Fb1, respectively). In contrast to the other HERVs, HML6-c14 transcripts localized to the nucleus and the average transcriptional level of HML6-c14 was higher in PIH placentas than in control placentas from normotensive pregnant women, although the differences did not reach significance ( p = 0.19). 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Psychology ; Gestational Age ; Human endogenous retrovirus ; Humans ; Hypertension, Pregnancy-Induced - etiology ; Hypertension, Pregnancy-Induced - genetics ; Hypertension, Pregnancy-Induced - metabolism ; Hypertensive disorder of pregnancy ; In situ hybridization ; Infant, Newborn ; Internal Medicine ; Male ; Obstetrics and Gynecology ; Organ Specificity ; Placenta ; Placenta - metabolism ; Pregnancy ; RNA, Messenger - metabolism ; TaqMan RT-PCR ; Term Birth - metabolism ; Tissue Distribution</subject><ispartof>Placenta (Eastbourne), 2008-03, Vol.29 (3), p.282-289</ispartof><rights>Elsevier Ltd</rights><rights>2007 Elsevier Ltd</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c451t-326d726d8f5f791fa2b3112af2d713b015e05c419363699ea87452d7229976923</citedby><cites>FETCH-LOGICAL-c451t-326d726d8f5f791fa2b3112af2d713b015e05c419363699ea87452d7229976923</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20181799$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18158183$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kudaka, W</creatorcontrib><creatorcontrib>Oda, T</creatorcontrib><creatorcontrib>Jinno, Y</creatorcontrib><creatorcontrib>Yoshimi, N</creatorcontrib><creatorcontrib>Aoki, Y</creatorcontrib><title>Cellular Localization of Placenta-Specific Human Endogenous Retrovirus (HERV) Transcripts and their Possible Implication in Pregnancy-Induced Hypertension</title><title>Placenta (Eastbourne)</title><addtitle>Placenta</addtitle><description>Abstract We previously investigated tissue specificity and temporal changes in expression of five human endogenous retroviruses (HERVs), including syncytin/ERVWE1 and syncytin 2 . 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Quantitative analysis showed that transcriptional levels of these four HERVs were lower in placentas obtained from pregnant women with PIH ( n = 22) than in those from normotensive pregnant women ( n = 87) and that the differences were statistically significant ( p = 0.001, 0.01, <0.001, and 0.04 for syncytin , syncytin 2 , HERV-H7/F(XA34), and HERV-Fb1, respectively). In contrast to the other HERVs, HML6-c14 transcripts localized to the nucleus and the average transcriptional level of HML6-c14 was higher in PIH placentas than in control placentas from normotensive pregnant women, although the differences did not reach significance ( p = 0.19). 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Teratology</topic><topic>Endogenous Retroviruses - genetics</topic><topic>Endogenous Retroviruses - metabolism</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gestational Age</topic><topic>Human endogenous retrovirus</topic><topic>Humans</topic><topic>Hypertension, Pregnancy-Induced - etiology</topic><topic>Hypertension, Pregnancy-Induced - genetics</topic><topic>Hypertension, Pregnancy-Induced - metabolism</topic><topic>Hypertensive disorder of pregnancy</topic><topic>In situ hybridization</topic><topic>Infant, Newborn</topic><topic>Internal Medicine</topic><topic>Male</topic><topic>Obstetrics and Gynecology</topic><topic>Organ Specificity</topic><topic>Placenta</topic><topic>Placenta - metabolism</topic><topic>Pregnancy</topic><topic>RNA, Messenger - metabolism</topic><topic>TaqMan RT-PCR</topic><topic>Term Birth - metabolism</topic><topic>Tissue Distribution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kudaka, W</creatorcontrib><creatorcontrib>Oda, T</creatorcontrib><creatorcontrib>Jinno, Y</creatorcontrib><creatorcontrib>Yoshimi, N</creatorcontrib><creatorcontrib>Aoki, Y</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Placenta (Eastbourne)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kudaka, W</au><au>Oda, T</au><au>Jinno, Y</au><au>Yoshimi, N</au><au>Aoki, Y</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cellular Localization of Placenta-Specific Human Endogenous Retrovirus (HERV) Transcripts and their Possible Implication in Pregnancy-Induced Hypertension</atitle><jtitle>Placenta (Eastbourne)</jtitle><addtitle>Placenta</addtitle><date>2008-03-01</date><risdate>2008</risdate><volume>29</volume><issue>3</issue><spage>282</spage><epage>289</epage><pages>282-289</pages><issn>0143-4004</issn><eissn>1532-3102</eissn><coden>PLACDF</coden><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>Abstract We previously investigated tissue specificity and temporal changes in expression of five human endogenous retroviruses (HERVs), including syncytin/ERVWE1 and syncytin 2 . In the current study, we examined the cellular localization and quantified the transcripts of five HERVs, syncytin , syncytin 2 , HERV-H7/F(XA34), HERV-Fb1, and HERV-HML6-c14, in order to elucidate their physiological and etiological roles in the placenta and in pregnancy-induced hypertension (PIH), respectively. In situ hybridization revealed trophoblast-specific transcription of all five HERVs. Consistent with a previous immunohistochemical analysis, syncytin 2 transcripts were detected only in cytotrophoblasts. All the HERVs except HERV-HML6-c14 (HML6-c14) were predominantly localized in the cytoplasm of syncytiotrophoblasts and/or cytotrophoblasts. Quantitative analysis showed that transcriptional levels of these four HERVs were lower in placentas obtained from pregnant women with PIH ( n = 22) than in those from normotensive pregnant women ( n = 87) and that the differences were statistically significant ( p = 0.001, 0.01, <0.001, and 0.04 for syncytin , syncytin 2 , HERV-H7/F(XA34), and HERV-Fb1, respectively). In contrast to the other HERVs, HML6-c14 transcripts localized to the nucleus and the average transcriptional level of HML6-c14 was higher in PIH placentas than in control placentas from normotensive pregnant women, although the differences did not reach significance ( p = 0.19). These results suggest that placenta-specific HERVs may have some function in the human placenta and that their reduced expression in PIH placentas is not merely a secondary effect derived from the pathology of PIH placentas.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>18158183</pmid><doi>10.1016/j.placenta.2007.11.009</doi><tpages>8</tpages></addata></record> |
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| subjects | Adult Biological and medical sciences Case-Control Studies Embryology: invertebrates and vertebrates. Teratology Endogenous Retroviruses - genetics Endogenous Retroviruses - metabolism Female Fundamental and applied biological sciences. Psychology Gestational Age Human endogenous retrovirus Humans Hypertension, Pregnancy-Induced - etiology Hypertension, Pregnancy-Induced - genetics Hypertension, Pregnancy-Induced - metabolism Hypertensive disorder of pregnancy In situ hybridization Infant, Newborn Internal Medicine Male Obstetrics and Gynecology Organ Specificity Placenta Placenta - metabolism Pregnancy RNA, Messenger - metabolism TaqMan RT-PCR Term Birth - metabolism Tissue Distribution |
| title | Cellular Localization of Placenta-Specific Human Endogenous Retrovirus (HERV) Transcripts and their Possible Implication in Pregnancy-Induced Hypertension |
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