Cellular Localization of Placenta-Specific Human Endogenous Retrovirus (HERV) Transcripts and their Possible Implication in Pregnancy-Induced Hypertension

Abstract We previously investigated tissue specificity and temporal changes in expression of five human endogenous retroviruses (HERVs), including syncytin/ERVWE1 and syncytin 2 . In the current study, we examined the cellular localization and quantified the transcripts of five HERVs, syncytin , syn...

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Bibliographic Details
Published in:Placenta (Eastbourne) 2008-03, Vol.29 (3), p.282-289
Main Authors: Kudaka, W, Oda, T, Jinno, Y, Yoshimi, N, Aoki, Y
Format: Article
Language:English
Subjects:
Adult
Biological and medical sciences
Case-Control Studies
Embryology: invertebrates and vertebrates. Teratology
Endogenous Retroviruses - genetics
Endogenous Retroviruses - metabolism
Female
Fundamental and applied biological sciences. Psychology
Gestational Age
Human endogenous retrovirus
Humans
Hypertension, Pregnancy-Induced - etiology
Hypertension, Pregnancy-Induced - genetics
Hypertension, Pregnancy-Induced - metabolism
Hypertensive disorder of pregnancy
In situ hybridization
Infant, Newborn
Internal Medicine
Male
Obstetrics and Gynecology
Organ Specificity
Placenta
Placenta - metabolism
Pregnancy
RNA, Messenger - metabolism
TaqMan RT-PCR
Term Birth - metabolism
Tissue Distribution
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Summary:Abstract We previously investigated tissue specificity and temporal changes in expression of five human endogenous retroviruses (HERVs), including syncytin/ERVWE1 and syncytin 2 . In the current study, we examined the cellular localization and quantified the transcripts of five HERVs, syncytin , syncytin 2 , HERV-H7/F(XA34), HERV-Fb1, and HERV-HML6-c14, in order to elucidate their physiological and etiological roles in the placenta and in pregnancy-induced hypertension (PIH), respectively. In situ hybridization revealed trophoblast-specific transcription of all five HERVs. Consistent with a previous immunohistochemical analysis, syncytin 2 transcripts were detected only in cytotrophoblasts. All the HERVs except HERV-HML6-c14 (HML6-c14) were predominantly localized in the cytoplasm of syncytiotrophoblasts and/or cytotrophoblasts. Quantitative analysis showed that transcriptional levels of these four HERVs were lower in placentas obtained from pregnant women with PIH ( n = 22) than in those from normotensive pregnant women ( n = 87) and that the differences were statistically significant ( p = 0.001, 0.01,
ISSN:0143-4004
1532-3102
DOI:10.1016/j.placenta.2007.11.009