Role of polymorphonuclear neutrophils (PMNs) and NK cells in the protection conferred by different vaccines against Chlamydophila abortus infection

Ovine enzootic abortion (OEA) is caused by Chlamydophila abortus, an intracellular bacterium which acts by infecting the placenta, causing abortion in the last term of gestation. The main prevention strategy against OEA is the vaccination of flocks. An effective vaccine against C. abortus must induc...

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Bibliographic Details
Published in:Research in veterinary science 2007-06, Vol.82 (3), p.314-322
Main Authors: Ortega, N., Caro, M.R., Buendía, A.J., Gallego, M.C., Del Río, L., Martínez, C.M., Nicolas, L., Cuello, F., Salinas, J.
Format: Article
Language:English
Subjects:
Abortion
abortion (animals)
adjuvant
aluminum hydroxide
Animals
Bacteria
bacterial infections
Bacterial Vaccines - immunology
cell-mediated immunity
Chlamydophila abortus
Chlamydophila Infections - immunology
Chlamydophila Infections - prevention & control
disease prevention
drug evaluation
Female
Immune system
immunologic factors
inactivated vaccines
Infections
Innate immune response
Killer Cells, Natural - immunology
live vaccines
Lymphocytes
Mice
Mice, Inbred BALB C
mortality
Mouse model
natural killer cells
neutrophils
Neutrophils - immunology
ovine enzootic abortion
protective effect
QS-21
saponins
sheep
sheep diseases
Time Factors
vaccination
Vaccine
vaccine adjuvants
Vaccines
Veterinary medicine
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Summary:Ovine enzootic abortion (OEA) is caused by Chlamydophila abortus, an intracellular bacterium which acts by infecting the placenta, causing abortion in the last term of gestation. The main prevention strategy against OEA is the vaccination of flocks. An effective vaccine against C. abortus must induce a Th1-like specific immune response, which is characterized by the early production of IFN-γ and the activation of CD8 +T cells. Moreover, vaccine effectiveness could be modulated by the functioning of the innate immunity. The purpose of this study was to ascertain how polymorphonuclear neutrophils (PMNs) and NK cells might influence vaccine-induced protection. The live attenuated 1B vaccine and two inactivated experimental vaccines, adjuvated with aluminium hydroxide (AH) or QS-21 (QS), were used in PMN-depleted or NK cell-depleted mice. For PMN depletion, RB6-8C5 monoclonal antibody, which recognizes GR1 +receptors (Robben, P.M., LaRegina, M., Kuziel, W.A., Sibley, L.D. 2005. Recruitment of Gr-1 + monocytes is essential for control of acute toxoplasmosis. The Journal of Experimental Medicine 201, 1761–1769.) was used, while for NK cell-depletion the anti-asialo GM1 polyclonal antibody was used. The depletion of PMNs caused 100% mortality in non-vaccinated mice (NV) and 60% mortality in the AH-vaccinated mice by day 10 p.i., while both groups showed a significant increase in their bacterial burden in the liver by day 4 p.i. The depletion of NK cells caused mortality only in the NV group (50% by day 10 p.i.), although this group and the 1B vaccinated mice showed an increased bacterial burden in the liver at day 4 p.i. Our results suggest that the importance of PMNs in inactivated vaccines depends on the adjuvant chosen. The results also demonstrated that the importance of NK cells is greater in live vaccines than in inactivated vaccines.
ISSN:0034-5288
1532-2661
DOI:10.1016/j.rvsc.2006.07.016