Enhanced Cellular Mobility Guided by TiO2 Nanotube Surfaces

The in vitro endothelial response of primary bovine aortic endothelial cells (BAECs) was investigated on a flat Ti surface vs a nanostructured TiO2 nanotube surface. The nanotopography provided nanoscale cues that facilitated cellular probing, cell sensing, and especially cell migration, where more...

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Bibliographic Details
Published in:Nano letters 2008-03, Vol.8 (3), p.786-793
Main Authors: Brammer, Karla S, Oh, Seunghan, Gallagher, John O, Jin, Sungho
Format: Article
Language:English
Subjects:
Actins - metabolism
Animals
Biological and medical sciences
Biotechnology
Cattle
Cell Movement
Cell Shape
Cells, Cultured
Condensed matter: structure, mechanical and thermal properties
Cross-disciplinary physics: materials science
rheology
Diffusion in nanoscale solids
Diffusion in solids
Endothelial Cells - cytology
Endothelial Cells - metabolism
Endothelin-1 - metabolism
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
Low-dimensional structures (superlattices, quantum well structures, multilayers): structure, and nonelectronic properties
Materials science
Methods. Procedures. Technologies
Microscopy, Electron, Scanning
Nanoscale materials and structures: fabrication and characterization
Nanotubes
Nanotubes - chemistry
Nanotubes - ultrastructure
Nitric Oxide - metabolism
Others
Physics
Surface Properties
Surfaces and interfaces
thin films and whiskers (structure and nonelectronic properties)
Titanium - metabolism
Transport properties of condensed matter (nonelectronic)
Various methods and equipments
Vinculin - metabolism
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Summary:The in vitro endothelial response of primary bovine aortic endothelial cells (BAECs) was investigated on a flat Ti surface vs a nanostructured TiO2 nanotube surface. The nanotopography provided nanoscale cues that facilitated cellular probing, cell sensing, and especially cell migration, where more organized actin cytoskeletal filaments formed lamellipodia and locomotive morphologies. Motile cell protrusions were able to probe down into the nanotube pores for contact stimulation, and focal adhesions were formed and disassembled readily for enhanced advancement of cellular fronts, which was not observed on a flat substrate of titanium. NO x and endothelin-1 functional assays confirmed that the nanotubes also up-regulated an antithrombic cellular state for maintaining vascular tone. The enhanced endothelial response to TiO2 nanotubes is significant for a potential modification of vascular stent surfaces in order to increase the rate and reliability of endothelialization and endothelial cell migration onto the stent for repairing arterial injury after activation.
ISSN:1530-6984
1530-6992
DOI:10.1021/nl072572o