Over-expression of genes coding for proline oxidase, riboflavin kinase, cytochrome c oxidase and an MFS transporter induced by acriflavin in Trichophyton rubrum

Acriflavin (3,6-acridinediamine) and other acridine derivatives act in both prokaryotic and eukaryotic cells at the level of DNA-coiling enzymes (topoisomerases) causing the stabilization of the enzyme-DNA cleavable complex. In order to better understand the mode of action of acriflavin, Differentia...

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Bibliographic Details
Published in:Medical mycology (Oxford) 2008-03, Vol.46 (2), p.135-139
Main Authors: Segato, Fernando, Nozawa, Sérgio R., Rossi, Antonio, Martinez-Rossi, Nilce M.
Format: Article
Language:English
Subjects:
Acriflavine - metabolism
Acriflavine - pharmacology
Electron Transport Complex I
Electron Transport Complex IV - genetics
Electron Transport Complex IV - metabolism
Gene Expression Regulation, Fungal - drug effects
Membrane Transport Proteins - genetics
Membrane Transport Proteins - metabolism
Phosphotransferases (Alcohol Group Acceptor) - genetics
Phosphotransferases (Alcohol Group Acceptor) - metabolism
Proline Oxidase - genetics
Proline Oxidase - metabolism
Reverse Transcriptase Polymerase Chain Reaction
Trichophyton - drug effects
Trichophyton - enzymology
Trichophyton - genetics
Trichophyton - metabolism
Trichophyton rubrum
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Summary:Acriflavin (3,6-acridinediamine) and other acridine derivatives act in both prokaryotic and eukaryotic cells at the level of DNA-coiling enzymes (topoisomerases) causing the stabilization of the enzyme-DNA cleavable complex. In order to better understand the mode of action of acriflavin, Differential Display RT-PCR was used to isolate transcripts specifically over-expressed during exposure of Trichophyton rubrum mycelia to this drug. Five transcripts, whose differential expressions were confirmed by Northern blotting, revealed genes not previously described in this dermatophyte. Functional grouping identified putative enzymes possibly involved in the mitochondrial respiratory electron-transport chain and in iron transport. These results may be relevant to our understanding of the molecular events involved in the stress response of T. rubrum to acriflavin.
ISSN:1369-3786
1460-2709
DOI:10.1080/13693780701742381