Stress-induced mobility of OPHIO1 and OPHIO2, DNA transposons of the Dutch elm disease fungi

The mobility of transposable elements (TEs) can contribute to genome plasticity, under- or over-expression of genes and ectopic recombination. The data collected in this study provide evidence of stress-induced mobility of OPHIO1 and OPHIO2 transposons, recently detected in Ophiostoma ulmi and O. no...

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Bibliographic Details
Published in:Fungal genetics and biology 2008-04, Vol.45 (4), p.565-578
Main Authors: Bouvet, Guillaume F., Jacobi, Volker, Plourde, Karine V., Bernier, Louis
Format: Article
Language:English
Subjects:
5' Untranslated Regions
Ascomycota - genetics
Ascomycota - radiation effects
Autonomous mobility
Base Sequence
Binding Sites
Ceratocystis ulmi
Cold Temperature
DNA
DNA Transposable Elements
DNA transposons
gene expression
genetic recombination
genetic variation
genome
heat shock proteins
Hot Temperature
hsp promoter
Incomplete transposition
Molecular Sequence Data
mutants
O. ulmi
OPHIO1
OPHIO2
Ophiostoma
Ophiostoma novo-ulmi
Ophiostoma ulmi
plant pathogenic fungi
plant stress
promoter regions
Promoter Regions, Genetic
Recombination, Genetic
RNA, Fungal - genetics
RNA, Messenger - genetics
Stresses
Transposases - biosynthesis
transposons
Ultraviolet Rays
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Summary:The mobility of transposable elements (TEs) can contribute to genome plasticity, under- or over-expression of genes and ectopic recombination. The data collected in this study provide evidence of stress-induced mobility of OPHIO1 and OPHIO2 transposons, recently detected in Ophiostoma ulmi and O. novo-ulmi, the causal agents of Dutch elm disease (DED). The analyses of OPHIO UTRs and TIRs indicated the presence of two potential binding site motifs and a heat shock protein ( hsp) promoter which could be involved in the mobility of OPHIO1 following a heat shock stress. The exact position of the hsp promoter was determined by 5′ RACE PCR. After confirmation of the expression by RT-PCR of both OPHIO1 and OPHIO2 transposases in the absence of stress factors, we tested two experimental procedures to induce mobility of OPHIO TEs: (1) an exogenous (cloned) copy of OPHIO1 was introduced into the O. novo-ulmi subsp. americana strain W2 ( OPHIO1 free strain) to give mutant strain W2:OPHIO1. After exposure of W2:OPHIO1 to a 55 °C heat shock treatment, some of the survivors showed signs of incomplete transposition (excision without reinsertion) of OPHIO1. (2) The O. novo-ulmi subsp. novo-ulmi strain AST27, introgressed from O. ulmi and carrying a distinct endogenous copy of OPHIO2 ( OPHIO2-int.), was subjected to a series of abiotic stress treatments. Although a promoter sequence could not be identified, both exposures to UV light and to a 4 °C cold treatment caused perfect excision of OPHIO2-int. In contrast to OPHIO1, heat shock stress did not induce OPHIO2-int. mobility. Taken together, these results allow us to hypothesize a potential interspecific invasion of OPHIO transposons due to their mobility in Ophiostoma spp.
ISSN:1087-1845
1096-0937
DOI:10.1016/j.fgb.2007.12.007