Quantitative demonstration of intrathecal synthesis of high affinity immunoglobulin G in herpes simplex encephalitis using affinity-mediated immunoblotting

Abstract Three paired serial samples of CSF and serum (from days 8, 13 and 22) were taken from a patient referred to the National Hospital for Neurology and Neurosurgery with what was duly confirmed as having herpes simplex encephalitis using PCR. The samples were investigated using affinity-mediate...

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Bibliographic Details
Published in:Journal of neuroimmunology 2007-04, Vol.185 (1), p.130-135
Main Authors: Chapman, Miles D, Thompson, Edward J, Candler, Paul M, Dale, Russell C, Church, Andrew J, Giovannoni, Gavin
Format: Article
Language:English
Subjects:
Affinity
Aged
Allergy and Immunology
Antibodies, Viral - biosynthesis
Antibodies, Viral - blood
Antibodies, Viral - cerebrospinal fluid
Antibody Affinity
Area Under Curve
DNA, Viral
Encephalitis, Herpes Simplex - cerebrospinal fluid
Encephalitis, Herpes Simplex - immunology
Female
Herpes simplex encephalitis
Herpesvirus 1, Human - immunology
Humans
IgG
Image Processing, Computer-Assisted
Immunoblotting - methods
Immunoglobulin G - biosynthesis
Immunoglobulin G - blood
Immunoglobulin G - cerebrospinal fluid
Neurology
Polymerase Chain Reaction
Thiocyanate
Time Factors
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Summary:Abstract Three paired serial samples of CSF and serum (from days 8, 13 and 22) were taken from a patient referred to the National Hospital for Neurology and Neurosurgery with what was duly confirmed as having herpes simplex encephalitis using PCR. The samples were investigated using affinity-mediated immunoblotting followed by incubation with sodium thiocyanate. Digitisation of the blots enabled further analysis. We showed that the clones of antigen-specific IgG, which were produced intrathecally, were of higher relative affinity than polyclonal antigen-specific IgG.
ISSN:0165-5728
1872-8421
DOI:10.1016/j.jneuroim.2007.01.002