The antagonistic effects of a combination of vancomycin and minocycline in Staphylococcus aureus with heterogeneous resistance to vancomycin

Abstract Some methicillin-resistant Staphylococcus aureus (MRSA) strains in which combinations of vancomycin (VCM) and β-lactam antibiotics show antagonism have recently emerged, and these strains are called β-lactaminduced VCM-resistant MRSA (BIVR). We examined whether various antibiotics exhibited...

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Published in:Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy 2008-02, Vol.14 (1), p.15-22
Main Authors: Oshiro, Tomoko, Ikeda-Dantsuji, Yurika, Nagayama, Ariaki, Nagasawa, Zenzo, Hanaki, Hideaki
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - pharmacology
Bacteriolysis
Drug Resistance, Multiple, Bacterial
Hematology, Oncology and Palliative Medicine
Humans
Infectious Diseases
Medical Microbiology
Medicine
Medicine & Public Health
Methicillin Resistance
Microbial Sensitivity Tests
Microscopy, Electron, Transmission
Minocycline - pharmacology
Original Article
Staphylococcal Infections - microbiology
Staphylococcus aureus
Staphylococcus aureus - classification
Staphylococcus aureus - cytology
Staphylococcus aureus - drug effects
Vancomycin - pharmacology
Vancomycin Resistance
Virology
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Summary:Abstract Some methicillin-resistant Staphylococcus aureus (MRSA) strains in which combinations of vancomycin (VCM) and β-lactam antibiotics show antagonism have recently emerged, and these strains are called β-lactaminduced VCM-resistant MRSA (BIVR). We examined whether various antibiotics exhibited an antagonistic effect with VCM when used against Mu3 and Fu10 (representative BIVR strains), using a simple agar disc method. Chloramphenicol, tetracyclines, macrolides, and lincosamides showed an antagonistic effect with VCM. We attempted to elucidate the antagonistic mechanism of a combination of VCM and minocycline (MINO) in BIVR strains. We determined the rates of autolysis, autolytic activities, and the change in morphology of Mu3 treated with a combination of VCM and MINO. We observed that Mu3 grown in a combination of VCM and MINO showed increasing rates of autolysis, and lower minimal bacteriolytic enzyme dose (MBD) values compared with Mu3 grown in VCM alone, but no cell wall thickening was observed. Taken together, these results suggest that cell wall thickening may not be essential in the increased resistance of BIVR strains. Our present data therefore suggest that these combination therapies of VCM with tetracyclines should be adopted with great care in order to prevent VCM treatment failure.
ISSN:1341-321X
1437-7780
DOI:10.1007/s10156-007-0569-9