Completion of Meiosis I of preovulatory oocytes and facilitation of preimplantation embryo development by glial cell line-derived neurotrophic factor

Optimal maturation of oocytes and successful development of preimplantation embryos is essential for reproduction. We performed DNA microarray analyses of ovarian transcripts and identified glial cell line-derived neurotrophic factor (GDNF) secreted by cumulus, granulosa, and theca cells as an ovari...

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Bibliographic Details
Published in:Developmental biology 2008-03, Vol.315 (1), p.189-202
Main Authors: Kawamura, Kazuhiro, Ye, Yinghui, Kawamura, Nanami, Jing, Li, Groenen, Peter, Sollewijn Gelpke, Maarten, Rauch, Rami, Hsueh, Aaron J.W., Tanaka, Toshinobu
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Apoptosis - drug effects
Blastocyst
Cell Proliferation - drug effects
Cells, Cultured
Cumulus Cells - cytology
Embryo, Mammalian - metabolism
Embryonic Development
Enzyme-Linked Immunosorbent Assay
Female
GDNF
GFRA1
Glial Cell Line-Derived Neurotrophic Factor - genetics
Glial Cell Line-Derived Neurotrophic Factor - pharmacology
Glial Cell Line-Derived Neurotrophic Factor - physiology
Humans
Immunohistochemistry
Meiosis - physiology
Mice
Mice, Inbred Strains
Oocyte
Oocytes - cytology
Oocytes - metabolism
Ovarian Follicle - cytology
Ovary - cytology
Oviducts - metabolism
Pregnancy
Recombinant Proteins - metabolism
Recombinant Proteins - pharmacology
Statistics as Topic
Uterus - metabolism
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Summary:Optimal maturation of oocytes and successful development of preimplantation embryos is essential for reproduction. We performed DNA microarray analyses of ovarian transcripts and identified glial cell line-derived neurotrophic factor (GDNF) secreted by cumulus, granulosa, and theca cells as an ovarian factor stimulated by the preovulatory LH/hCG surge. Treatment of cumulus–oocyte complexes with GDNF enhanced first polar body extrusion with increase in cyclin B1 synthesis and the GDNF actions are likely mediated by its receptor GDNF family receptor-alpha1 (GFRA1) and a co-receptor ret proto-oncogene (Ret), both expressed in oocytes. However, treatment with GDNF did not affect germinal vesicle breakdown and cytoplasmic maturation of oocytes. During the preimplantation stages, GDNF was expressed in pregnant oviducts and uteri, whereas GFRA1 and Ret were expressed in embryos throughout early development with an increase after the early blastocyst stage. In blastocysts, both GDNF and GFRA1 were exclusively localized in trophectoderm cells, whereas Ret was detected in both cell lineages. Treatment with GDNF promoted the development of two-cell-stage embryos into blastocysts showing increased cell proliferation and decreased apoptosis mainly in trophectoderm cells. Our findings suggest potential paracrine roles of GDNF in the promotion of completion of meiosis I and the development of early embryos.
ISSN:0012-1606
1095-564X
DOI:10.1016/j.ydbio.2007.12.029