Preservation of the pHi during ischemia via PKC by intermittent hypoxia

In intermittent hypoxia adaptation (IHA) rat cardiomyocytes, the relationship between activated protein kinase C and intracellular acidification regulation during ischemia-reperfusion (I/R) was tested. Using [H(+)] indicator BCECF-AM, we analyzed the alterations of intracellular pH (pH(i)) in normox...

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Published in:Biochemical and biophysical research communications 2007-05, Vol.356 (2), p.329-333
Main Authors: Li, Jun, Zhang, Hao, Zhu, Wei-Zhong, Yu, Zhuo, Guo, Ang, Yang, Huang-Tian, Zhou, Zhao-Nian
Format: Article
Language:English
Subjects:
Adaptation, Physiological
Animals
Disease Models, Animal
Hydrogen-Ion Concentration
Hypoxia - enzymology
Hypoxia - physiopathology
Ischemia - physiopathology
Male
Protein Kinase C - physiology
Rats
Rats, Sprague-Dawley
Reperfusion
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Summary:In intermittent hypoxia adaptation (IHA) rat cardiomyocytes, the relationship between activated protein kinase C and intracellular acidification regulation during ischemia-reperfusion (I/R) was tested. Using [H(+)] indicator BCECF-AM, we analyzed the alterations of intracellular pH (pH(i)) in normoxia and IHA rat cardiomyocytes during I/R. With the time of ischemia, the pH(i) decreased progressively in normal cardiomyocytes, but fewer alterations in IHA myocytes. Treatment of IHA and normoxia cardiomyocytes with 5 microM chelerythrine delayed the pH(i) recovery during post-ischemia. In contrast, the application of 1 microM phorbol 12-myristate 13-acetate in normoxia cardiomyocytes preserved the pH(i) during I/R, which was similar to that in IHA cardiomyocytes. Our data suggest that the stable PKC activation might contribute to preservation of the pH(i), which may be beneficial to maintain cardiac function during I/R in IHA rats.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2007.02.128