Afforestation of moorland leads to changes in crenarchaeal community structure

Group 1 Crenarchaeota are an abundant component of soil microbial communities. A number of distinct lineages have been discovered, and the Group 1.1b lineage is present in most soil types. Others, such as the Group 1.1c lineage, may be restricted to specific soil types, such as acidic forest soils....

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Bibliographic Details
Published in:	FEMS microbiology ecology 2007-04, Vol.60 (1), p.51-59
Main Authors:	Nicol, Graeme W, Campbell, Colin D, Chapman, Stephen J, Prosser, James I
Format:	Article
Language:	eng
Subjects:	16S rRNA Acidic soils Afforestation Archaea Bacteriology Biological and medical sciences Calluna - growth & development Cloning Community structure Coniferous forests Crenarchaeota Crenarchaeota - classification Crenarchaeota - genetics Crenarchaeota - growth & development DNA, Archaeal - analysis DNA, Archaeal - isolation & purification DNA, Ribosomal - analysis Ecosystem Electrophoresis Electrophoresis, Polyacrylamide Gel - methods Environmental changes forest soil Forest soils Forests Fundamental and applied biological sciences. Psychology Gel electrophoresis Microbial activity Microbiology Microorganisms Miscellaneous Molecular Sequence Data Moorland Phylogeny Pinus sylvestris Polymerase Chain Reaction Reaction products Reverse Transcriptase Polymerase Chain Reaction RNA, Ribosomal, 16S RNA-directed DNA polymerase rRNA 16S Sequence Analysis, DNA Soil Microbiology Soil structure Soil types Soils Trees
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Summary:	Group 1 Crenarchaeota are an abundant component of soil microbial communities. A number of distinct lineages have been discovered, and the Group 1.1b lineage is present in most soil types. Others, such as the Group 1.1c lineage, may be restricted to specific soil types, such as acidic forest soils. To determine the effects of afforestation on the Archaea of moorland soils, archaeal community structure was examined across three parallel 180 m transects, running from open heather moorland into a Scots pine forest. Communities were characterized using a combination of cloning and denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA reverse transcriptase-polymerase chain reaction products. Although forest soils selected for a reproducibly distinct crenarchaeal community structure from moorland soils, both habitats contained similar populations, indicated by comigrating DGGE bands. Archaeal communities in soils of both ecosystems were dominated by Group 1.1c crenarchaea, and reproducible differences in community structure within this lineage were observed between forest and moorland soils. The findings indicate that the afforestation of moorland soils can lead to changes in crenarchaeal community structure with a potential impact on ecosystem function.
ISSN:	0168-6496 1574-6941