Skeletal myogenesis on highly orientated microfibrous polyesterurethane scaffolds

Skeletal myogenesis is a complex process, which is known to be intimately depending on an optimal outside‐in substrate‐cell signaling. Current attempts to reproduce skeletal muscle tissue in vitro using traditional scaffolds mainly suffer from poor directionality of the myofibers, resulting in an in...

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Bibliographic Details
Published in:Journal of biomedical materials research. Part A 2008-03, Vol.84A (4), p.1094-1101
Main Authors: Riboldi, S.A., Sadr, N., Pigini, L., Neuenschwander, P., Simonet, M., Mognol, P., Sampaolesi, M., Cossu, G., Mantero, S.
Format: Article
Language:English
Subjects:
Animals
Biocompatible Materials - chemistry
Cell Adhesion
Cell Line
electrospinning
Equipment Design
fiber orientation
Materials Testing
Mice
Muscle Development
Muscle, Skeletal - metabolism
Myoblasts - metabolism
myogenesis
Polyesters - chemistry
Polyurethanes - chemistry
Rats
skeletal muscle
tissue engineering
Tissue Engineering - methods
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Summary:Skeletal myogenesis is a complex process, which is known to be intimately depending on an optimal outside‐in substrate‐cell signaling. Current attempts to reproduce skeletal muscle tissue in vitro using traditional scaffolds mainly suffer from poor directionality of the myofibers, resulting in an ineffective vectorial power generation. In this study, we aimed at investigating skeletal myogenesis on novel biodegradable microfibrous scaffolds made of DegraPol®, a block polyesterurethane previously demonstrated to be suitable for this application. DegraPol® was processed by electrospinning in the form of highly orientated (“O”) and nonorientated (“N/O”) microfibrous meshes and by solvent‐casting in the form of nonporous films (“F”). The effect of the fiber orientation at the scaffold surface was evaluated by investigating C2C12 and L6 proliferation (via SEM analysis and alamarBlue™ test) and differentiation (via RT‐PCR analysis and MHC immunostaining). We demonstrated that highly orientated elastomeric microfibrous DegraPol® scaffolds enable skeletal myogenesis in vitro by aiding in (a) myoblast adhesion, (b) myotube alignment, and (c) noncoplanar arrangement of cells, by providing the necessary directional cues along with architectural and mechanical support. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008
ISSN:1549-3296
1552-4965
DOI:10.1002/jbm.a.31534