Evaluation of various derivatization approaches for gas chromatography–mass spectrometry analysis of buprenorphine and norbuprenorphine

Various chemical derivatization approaches have been adapted for the analysis of buprenorphine and its major metabolite (norbuprenorphine) by GC–MS based methodologies. These approaches included alkylation, acylation, and silylation resulting in the formation of methyl, acetyl, trifluoroacetyl, pent...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Chromatography A 2008-02, Vol.1182 (1), p.93-112
Main Authors: Wu, Chih-Hung, Yang, Shu-Ching, Wang, Yu-Shan, Chen, Bud-Gen, Lin, Ching-Chiang, Liu, Ray H.
Format: Article
Language:English
Subjects:
Acylation
Alkylation
Analysis
Biological and medical sciences
Buprenorphine
Buprenorphine - analogs & derivatives
Buprenorphine - chemistry
Derivatization
Gas Chromatography-Mass Spectrometry - methods
GC–MS
General pharmacology
Medical sciences
Molecular Structure
Pharmacology. Drug treatments
Silylation
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Various chemical derivatization approaches have been adapted for the analysis of buprenorphine and its major metabolite (norbuprenorphine) by GC–MS based methodologies. These approaches included alkylation, acylation, and silylation resulting in the formation of methyl, acetyl, trifluoroacetyl, pentafluoropropionyl, heptafluorobutyryl, and trimethylsilyl derivatives. This study conducted a comprehensive evaluation on the merits of these approaches based on the following criteria: reaction yields and ionization efficiency of the derivatization products; chromatographic characteristics; and cross-contributions to the intensities of ions designating the analytes and the internal standards. Under acidic derivatization conditions, the analytes could form three artifact products. Overall, derivatization by acetyl anhydride resulted in best performance characteristics.
ISSN:0021-9673
DOI:10.1016/j.chroma.2007.11.063