Microarray analysis of hepatic genes differentially expressed in the presence of the unsaponifiable fraction of olive oil in apolipoprotein E-deficient mice

The hypothesis that the unsaponifiable fraction of olive oil dramatically influences hepatic gene expression was tested in mice. Two olive oils, obtained from the same olive cultivar but by different technological procedures, were characterized to show that they differed mainly in terms of the compo...

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Bibliographic Details
Published in:British journal of nutrition 2007-04, Vol.97 (4), p.628-638
Main Authors: Acín, Sergio, Navarro, María A., Perona, Javier S., Surra, Joaquín C., Guillen, Natalia, Arnal, Carmen, Sarría, Alfonso J., Arbonés-Mainar, José M., Carnicer, Ricardo, Ruiz-Gutiérrez, Valentina, Osada, Jesús
Format: Article
Language:English
Subjects:
Alzheimer's disease
animal models
Animals
Apolipoprotein E-deficient mice
apolipoproteins
Apolipoproteins E - deficiency
Biological and medical sciences
biomarkers
cholesteremic effect
Cholesterol
cholesterol metabolism
Diet
Dietary Fats, Unsaturated - pharmacology
Fatty acids
Fatty Acids - analysis
Feeding. Feeding behavior
Food Handling - methods
Fundamental and applied biological sciences. Psychology
Gene expression
gene expression regulation
Gene Expression Regulation - drug effects
gene overexpression
genes
High density lipoprotein
Lipids
Liver
Liver - metabolism
Male
Mice
Mice, Inbred C57BL
Mice, Knockout
Microarray Analysis
microarray technology
monounsaturated fatty acids
nutrition-genotype interaction
Olive Oil
Plant Oils - chemistry
Plant Oils - pharmacology
Plasma
reverse transcriptase polymerase chain reaction
Reverse Transcriptase Polymerase Chain Reaction - methods
RNA, Messenger - genetics
saponification
Unsaponifiable fraction
unsaponifiable fractions
Vertebrates: anatomy and physiology, studies on body, several organs or systems
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Summary:The hypothesis that the unsaponifiable fraction of olive oil dramatically influences hepatic gene expression was tested in mice. Two olive oils, obtained from the same olive cultivar but by different technological procedures, were characterized to show that they differed mainly in terms of the composition/quantity of this unsaponifiable fraction. Using DNA microarrays, hepatic gene expression was analysed in apoE-deficient mice fed one of two isoenergetic, isonitrogenous diets containing either 10 % (w/w) olive oil or unsaponifiable fraction-enriched olive oil. To provide an initial screening of potential candidate genes involved in a differential response, only genes with remarkably modified expression (signal log2 ratio >3 or
ISSN:0007-1145
1475-2662
DOI:10.1017/S0007114507657912