Las-like quorum-sensing system negatively regulates both pyoluteorin and phenazine-1-carboxylic acid production in Pseudomonas sp. M18

A las -like quorum-sensing system in Pseudomonas sp. M18 was identified, which consisted of lasI and lasR genes encoding LuxI-LuxR type regulator. Several functions of the las system from strain M18 were investigated in this study. The chromosomal inactivation of either lasI or lasR by recombination...

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Bibliographic Details
Published in:Science China. Life sciences 2008-02, Vol.51 (2), p.174-181
Main Authors: Chen, Yun, Wang, XiaoLei, Huang, XianQing, Zhang, XueHong, Xu, YuQuan
Format: Article
Language:English
Subjects:
Bacterial Physiological Phenomena
Bacterial Proteins - isolation & purification
Bacterial Proteins - physiology
Biomedical and Life Sciences
DNA-Binding Proteins - isolation & purification
DNA-Binding Proteins - physiology
Down-Regulation - physiology
Gene Expression Regulation, Bacterial - physiology
Growth Inhibitors - physiology
Life Sciences
Movement - physiology
Phenazines - antagonists & inhibitors
Phenazines - metabolism
Phenols - antagonists & inhibitors
Phenols - metabolism
Pseudomonas
Pseudomonas - genetics
Pseudomonas - growth & development
Pseudomonas - metabolism
Pyrroles - antagonists & inhibitors
Pyrroles - metabolism
Quorum Sensing - physiology
Trans-Activators - isolation & purification
Trans-Activators - physiology
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Summary:A las -like quorum-sensing system in Pseudomonas sp. M18 was identified, which consisted of lasI and lasR genes encoding LuxI-LuxR type regulator. Several functions of the las system from strain M18 were investigated in this study. The chromosomal inactivation of either lasI or lasR by recombination increased the production of both pyoluteorin (Plt) and phenazine-1-carboxylic acid (PCA) by 4–5 fold and 2–3 fold over that of the wild type strain of M18, respectively. Production of both antibiotics was restored to wild-type levels after in trans complementation with the wild-type lasI or lasR gene. Expression of the translational fusions pltA′-′lacZ and phzA′-′lacZ further confirmed the negative effect of lasI or lasR on both biosynthetic operons, and it was also demonstrated that the las system was related to the ability of swarming motility and the inhibition of cell growth.
ISSN:1006-9305
1674-7305
1862-2798
1869-1889
DOI:10.1007/s11427-008-0026-8