Biocompatibility of poly( d , l -lactide- co -glycolide) nanoparticles conjugated with alendronate

Abstract Nanoparticles made of a conjugate of poly( d , l -lactide- co -glycolide) with alendronate (PLGA–ALE NPs), were prepared by emulsion/solvent evaporation technique. The conjugation yield, determined by MALDI TOF analysis, was 30–35%. PLGA–ALE NPs size, evaluated by photon correlation spectro...

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Published in:Biomaterials 2008-04, Vol.29 (10), p.1400-1411
Main Authors: Cenni, Elisabetta, Granchi, Donatella, Avnet, Sofia, Fotia, Caterina, Salerno, Manuela, Micieli, Dorotea, Sarpietro, Maria G, Pignatello, Rosario, Castelli, Francesco, Baldini, Nicola
Format: Article
Language:English
Subjects:
Advanced Basic Science
Alendronate - chemistry
Alkaline Phosphatase - metabolism
Bisphosphonate
Blood compatibility
Cell Survival - drug effects
Dentistry
Drug delivery
Hemolysis - drug effects
Humans
Lactic Acid - chemistry
Leukocyte Count
Microscopy, Electron, Scanning
Nanoparticle
Nanoparticles
Platelet Activation - drug effects
Poly(lactic- co-glycolic acid) copolymer
Polyglycolic Acid - chemistry
Polymers - chemistry
Polymers - pharmacology
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Summary:Abstract Nanoparticles made of a conjugate of poly( d , l -lactide- co -glycolide) with alendronate (PLGA–ALE NPs), were prepared by emulsion/solvent evaporation technique. The conjugation yield, determined by MALDI TOF analysis, was 30–35%. PLGA–ALE NPs size, evaluated by photon correlation spectroscopy, was 198.7 ± 0.2 nm. Haemocompatibility studies using different concentrations of PLGA–ALE NPs did not show any significant effect on haemolysis, leukocyte number, platelet activation, APTT and complement consumption, in comparison with blood incubated with phosphate buffered saline (PBS). A significant reduction of the prothrombin activity was demonstrated after incubation with 560 μg/ml of PLGA–ALE NPs; a significant increase was observed at the highest dilutions. The viability of human umbilical vein endothelial cells and bone marrow stromal cells (BMSC), evaluated through the neutral red test, was not affected by PLGA–ALE NPs. There were no significant differences in cell-associated alkaline phosphatase between BMSC incubated with PLGA–ALE NP- and PBS-added media. These results demonstrated that PLGA–ALE NPs had an acceptable degree of blood compatibility and were not cytotoxic; therefore, they may be considered suitable for intravenous administration.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2007.12.022