Chicory increases acetate turnover, but not propionate and butyrate peripheral turnovers in rats

Chicory roots are rich in inulin that is degraded into SCFA in the caecum and colon. Whole-body SCFA metabolism was investigated in rats during food deprivation and postprandial states. After 22 h of food deprivation, sixteen rats received an IV injection of radioactive 14C-labelled SCFA. The volume...

Full description

Saved in:
Bibliographic Details
Published in:British journal of nutrition 2008-02, Vol.99 (2), p.287-296
Main Authors: Pouteau, Etienne, Rochat, Florence, Jann, Alfred, Meirim, Isabelle, Sanchez-Garcia, Jose-Luis, Ornstein, Kurt, German, Bruce, Ballèvre, Olivier
Format: Article
Language:English
Subjects:
Acetates - blood
acetic acid
animal models
Animals
blood chemistry
blood lipids
Butyrates - blood
butyric acid
Carbon Radioisotopes
cecum
Cecum - metabolism
Chicory
Cichorium intybus
colon
Colon - metabolism
experimental design
Fatty Acids, Volatile - biosynthesis
Fatty Acids, Volatile - blood
feed deprivation
Female
Food Deprivation - physiology
Half-Life
inulin
isotope labeling
Kinetics
lipid metabolism
Male
Portal Vein - metabolism
Postprandial Period - physiology
prebiotics
Propionates - blood
propionic acid
Rats
Short-chain fatty acids
tissue weight
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Chicory roots are rich in inulin that is degraded into SCFA in the caecum and colon. Whole-body SCFA metabolism was investigated in rats during food deprivation and postprandial states. After 22 h of food deprivation, sixteen rats received an IV injection of radioactive 14C-labelled SCFA. The volume of distribution and the fractional clearance rate of SCFA were 0·25–0·27 litres/kg and 5·4–5·9 %/min, respectively. The half-life in the first extracellular rapidly decaying compartment was between 0·9 and 1·4 min. After 22 h of food deprivation, another seventeen rats received a primed continuous IV infusion of 13C-labelled SCFA for 2 h. Isotope enrichment (13C) of SCFA was determined in peripheral arterial blood by MS. Peripheral acetate, propionate and butyrate turnover rates were 29, 4 and 0·3 μmol/kg per min respectively. Following 4 weeks of treatment with chicory root or control diets, eighteen fed rats received a primed continuous IV infusion of 13C-labelled SCFA for 2 h. Intestinal degradation of dietary chicory lowered caecal pH, enhanced caecal and colonic weights, caecal SCFA concentrations and breath H2.The diet with chicory supplementation enhanced peripheral acetate turnover by 25 % (P = 0·017) concomitant with an increase in plasma acetate concentration. There were no changes in propionate or butyrate turnovers. In conclusion, by setting up a multi-tracer approach to simultaneously assess the turnovers of acetate, propionate and butyrate it was demonstrated that a chronic chicory-rich diet significantly increases peripheral acetate turnover but not that of propionate or butyrate in rats.
ISSN:0007-1145
1475-2662
DOI:10.1017/S0007114507815790