Suppression of lipopolysaccharide-induced of inducible nitric oxide synthase and cyclooxygenase-2 by Sanguis Draconis, a dragon's blood resin, in RAW 264.7 cells

Sanguis Draconis (SD) is a kind of dragon's blood resin that is obtained from Daemomorops draco (Palmae). It is used in traditional medicine and has shown anti-inflammatory activity in some diseases. In this study, we examined the effects of Sanguis Dranonis ethanol extract (SDEE) on LPS-induce...

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Bibliographic Details
Published in:Journal of ethnopharmacology 2008-02, Vol.115 (3), p.455-462
Main Authors: Choy, Cheuk-Sing, Hu, Chien-Ming, Chiu, Wen-Ta, Lam, Carlos-Shu Kei, Ting, Yih, Tsai, Shin-Han, Wang, Tzu-Chien
Format: Article
Language:English
Subjects:
Animals
anti-inflammatory activity
Anti-Inflammatory Agents - isolation & purification
Anti-Inflammatory Agents - pharmacology
Antioxidants - isolation & purification
Antioxidants - pharmacology
Arecaceae - chemistry
Biological and medical sciences
Cell Line
cell lines
cultured cells
Cyclooxygenase 2 - drug effects
Cyclooxygenase 2 - metabolism
Cyclooxygenase-2
Daemonorops
Daemonorops draco
Dinoprostone - biosynthesis
Draco
enzyme activity
enzyme inhibitors
gene expression
Gene Expression Regulation - drug effects
General pharmacology
Heme Oxygenase-1 - drug effects
Heme Oxygenase-1 - metabolism
Inducible nitric oxide synthase
inflammation
Inflammation - chemically induced
Inflammation - drug therapy
Lipopolysaccharide
Lipopolysaccharides
macrophages
Macrophages - drug effects
Macrophages - metabolism
mechanism of action
Medical sciences
medicinal plants
medicinal properties
Medicine, Traditional
Mice
nitric oxide
Nitric Oxide - biosynthesis
nitric oxide synthase
Nitric Oxide Synthase Type II - drug effects
Nitric Oxide Synthase Type II - metabolism
Nuclear factor-κB
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
Plant Extracts - pharmacology
plant exudates
prostaglandin synthase
prostaglandins
Resins, Plant
Sanguis Draconis
transcription factors
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Summary:Sanguis Draconis (SD) is a kind of dragon's blood resin that is obtained from Daemomorops draco (Palmae). It is used in traditional medicine and has shown anti-inflammatory activity in some diseases. In this study, we examined the effects of Sanguis Dranonis ethanol extract (SDEE) on LPS-induced inflammation using RAW 264.7 cells. Our data indicated that SDEE inhibits LPS-stimulated NO, PGE 2, IL-1β and TNF-α release, and iNOS and COX-2 expression. Furthermore, SDEE suppressed the LPS-induced p65 expression of NF-κB, which was associated with the inhibition of IκB-α degradation. We also found that the expression of HO-1 was significantly increased in RAW 264.7 cells by SDEE. These results suggest among possibilities of anti-inflammation that SDEE inhibits the production of NO and PGE 2 by the down-regulation of iNOS and COX-2 gene expression via the suppression of NF-κB (p65) activation. SDEE can induce HO-1 over-expression in macrophage cells, which indicates that it may possess antioxidant properties. This result means that SEDD its anti-inflammatory effects in macrophages may be through a novel mechanism that involves the action of HO-1. Thus, SD could provide a potential therapeutic approach for inflammation-associated disorders.
ISSN:0378-8741
1872-7573
DOI:10.1016/j.jep.2007.10.012