Multifaceted transcriptional regulation of the murine intestinal mucus layer by endogenous microbiota

The intestinal mucus layer and endogenous microbiota are strongly intertwined and this contributes to the maintenance of the epithelial barrier and ultimately of gut homeostasis. To understand the molecular foundations of such relationship, we investigated if the nature of the microbiota transcripti...

Full description

Saved in:
Bibliographic Details
Published in:Genomics (San Diego, Calif.) Calif.), 2008, Vol.91 (1), p.70-77
Main Authors: Comelli, Elena M., Simmering, Rainer, Faure, Magali, Donnicola, Dominique, Mansourian, Robert, Rochat, Florence, Corthesy-Theulaz, Irène, Cherbut, Christine
Format: Article
Language:English
Subjects:
Adult
Animals
Bacteria
Biological and medical sciences
Colon - metabolism
Colon - microbiology
Cytoskeleton
Down-Regulation - physiology
Fundamental and applied biological sciences. Psychology
Gene Expression Profiling
Genes. Genome
Genetics of eukaryotes. Biological and molecular evolution
Germ-Free Life - physiology
Humans
Ileum - metabolism
Ileum - microbiology
Infant
Infant, Newborn
Intestinal Mucosa - metabolism
Intestinal Mucosa - microbiology
Intestine
Male
Mice
Mice, Inbred C3H
Microarray analysis
Molecular and cellular biology
Molecular genetics
Mucin
Mucins - biosynthesis
Mucins - genetics
Oligonucleotide Array Sequence Analysis
Transcription. Transcription factor. Splicing. Rna processing
Trefoil Factor-3
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The intestinal mucus layer and endogenous microbiota are strongly intertwined and this contributes to the maintenance of the epithelial barrier and ultimately of gut homeostasis. To understand the molecular foundations of such relationship, we investigated if the nature of the microbiota transcriptionally regulates mucus layer composition in vivo. We found that the expression of mucins 1 to 4 and trefoil factor 3 was down-regulated in the ileum and colon of conventional and reconventionalized mice compared with germ-free animals. Conversely, very limited colon-restricted changes in transmembrane mucins were detected in mice colonized with human adult or baby microbiota. Moreover, by microarray analysis, the murine endogenous microbiota was found to modulate genes putatively involved in mucin secretion. These findings show that a well-established microbial community participates in the regulation of the gut mucus layer and that its composition and adequacy to the host are key factors in this process.
ISSN:0888-7543
1089-8646
DOI:10.1016/j.ygeno.2007.09.006