Inferring ancestral origin using a single multiplex assay of ancestry-informative marker SNPs

Abstract Tests that infer the ancestral origin of a DNA sample have considerable potential in the development of forensic tools that can help to guide crime investigation. We have developed a single-tube 34-plex SNP assay for the assignment of ancestral origin by choosing ancestry-informative marker...

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Bibliographic Details
Published in:Forensic science international : genetics 2007-12, Vol.1 (3), p.273-280
Main Authors: Phillips, C, Salas, A, Sánchez, J.J, Fondevila, M, Gómez-Tato, A, Álvarez-Dios, J, Calaza, M, de Cal, M. Casares, Ballard, D, Lareu, M.V, Carracedo, Á
Format: Article
Language:English
Subjects:
African Continental Ancestry Group - genetics
Algorithms
Alleles
Asian Continental Ancestry Group - genetics
Databases, Nucleic Acid
DNA - genetics
DNA - isolation & purification
European Continental Ancestry Group - genetics
Forensic Genetics - methods
Forensic Genetics - statistics & numerical data
Gene Frequency
Genetics, Population - methods
Genetics, Population - statistics & numerical data
Humans
Pathology
Polymorphism, Single Nucleotide
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Summary:Abstract Tests that infer the ancestral origin of a DNA sample have considerable potential in the development of forensic tools that can help to guide crime investigation. We have developed a single-tube 34-plex SNP assay for the assignment of ancestral origin by choosing ancestry-informative markers (AIMs) exhibiting highly contrasting allele frequency distributions between the three major population-groups. To predict ancestral origin from the profiles obtained, a classification algorithm was developed based on maximum likelihood. Sampling of two populations each from African, European and East Asian groups provided training sets for the algorithm and this was tested using the CEPH Human Genome Diversity Panel. We detected negligible theoretical and practical error for assignments to one of the three groups analyzed with consistently high classification probabilities, even when using reduced subsets of SNPs. This study shows that by choosing SNPs exhibiting marked allele frequency differences between population-groups a practical forensic test for assigning the most likely ancestry can be achieved from a single multiplexed assay.
ISSN:1872-4973
1878-0326
DOI:10.1016/j.fsigen.2007.06.008