Towards functional characterisation of the members of the R2R3‐MYB gene family from Arabidopsis thaliana

Summary Transcription factors containing a conserved DNA‐binding domain similar to that of the proto‐oncogene c‐myb have been identified in nearly all eukaryotes. MYB‐related proteins from plants generally contain two related helix‐turn‐helix motifs, the R2 and R3 repeats. It was estimated that Arab...

Full description

Saved in:
Bibliographic Details
Published in:The Plant journal : for cell and molecular biology 1998-10, Vol.16 (2), p.263-276
Main Authors: Kranz, Harald D., Denekamp, Marten, Greco, Raffaella, Jin, Hailing, Leyva, Antonio, Meissner, Ruth C., Petroni, Katia, Urzainqui, Ana, Bevan, Michael, Martin, Cathie, Smeekens, Sjef, Tonelli, Chiara, Paz‐Ares, Javier, Weisshaar, Bernd
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Arabidopsis - genetics
Arabidopsis Proteins - genetics
Arabidopsis thaliana
Biological and medical sciences
Chromosome Mapping
Chromosomes
DNA-Binding Proteins - genetics
Fundamental and applied biological sciences. Psychology
Genome, Plant
Helix-Turn-Helix Motifs - genetics
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Plant Proteins - genetics
Polymerase Chain Reaction
Proto-Oncogene Proteins c-myb
Transcription Factors - genetics
Transcription. Transcription factor. Splicing. Rna processing
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Summary Transcription factors containing a conserved DNA‐binding domain similar to that of the proto‐oncogene c‐myb have been identified in nearly all eukaryotes. MYB‐related proteins from plants generally contain two related helix‐turn‐helix motifs, the R2 and R3 repeats. It was estimated that Arabidopsis thaliana contains more than 100 R2R3‐MYB genes. The few cases where functional data are available suggest an important role of these genes in the regulation of secondary metabolism, the control of cell shape, disease resistance, and hormone responses. To determine the full regulatory potential of this large family of regulatory genes, a systematic search for the function of all genes of this family was initiated . Sequence data for more than 90 different A. thaliana R2R3‐MYB genes have been obtained. Sequence comparison revealed conserved amino acid motifs shared by subgroups of R2R3‐MYB genes in addition to the characteristic DNA‐binding domain. No significant clustering of the genes was detected, although they are not uniformly distributed throughout the A. thaliana genome. R2R3‐MYB gene expression levels were determined under more than 20 different growth conditions including hormone treatment, infection with pathogens and various stress conditions. MYB genes are specifically expressed in different tissues and physiological conditions, indicating the potential for involvement in various regulatory processes. The sequence and expression data together with the map positions of nearly all MYB genes in A. thaliana provide a substantial basis for further studies of this important group of transcription factors.
ISSN:0960-7412
1365-313X
DOI:10.1046/j.1365-313x.1998.00278.x