T cell receptor β-chain repertoire in inclusion body myositis

Inclusion body myositis (IBM) is the most common muscle disease affecting individuals over 50 years of age. The inflammatory reaction is characterized by cell infiltrates predominated by CD8 + cytotoxic T cells. To analyze clonality of muscle infiltrating lymphocytes, we studied the complementarity...

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Bibliographic Details
Published in:Journal of neuroimmunology 1998-11, Vol.91 (1), p.129-134
Main Authors: Fyhr, Ing-Marie, Moslemi, Ali-Reza, Lindberg, Christopher, Oldfors, Anders
Format: Article
Language:English
Subjects:
Aged
Aged, 80 and over
Biopsy
Complementarity determining region 3
DNA Primers
DNA, Complementary
Female
Humans
Immunoglobulin Variable Region - genetics
Immunoglobulin Variable Region - immunology
Inclusion Bodies - immunology
Inclusion Bodies - pathology
Inclusion body myositis
Male
Middle Aged
Myositis - immunology
Myositis - pathology
Receptors, Antigen, T-Cell, alpha-beta - genetics
Receptors, Antigen, T-Cell, alpha-beta - immunology
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - analysis
T cell receptor
T-Lymphocytes - chemistry
T-Lymphocytes - immunology
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Summary:Inclusion body myositis (IBM) is the most common muscle disease affecting individuals over 50 years of age. The inflammatory reaction is characterized by cell infiltrates predominated by CD8 + cytotoxic T cells. To analyze clonality of muscle infiltrating lymphocytes, we studied the complementarity determining region 3 (CDR3) length distribution of the T cell receptor (TCR). Muscle infiltrating lymphocytes were studied in three IBM patients and compared with peripheral blood lymphocytes (PBL) in two of these patients. The study was performed by reverse transcription polymerase chain reaction (RT-PCR) of RNA extracted from muscle tissue and PBL followed by analysis of fragment length distribution of the CDR3 region in each of 24 different Vβ families. There was a restricted usage of TCR Vβ gene families in muscle infiltrating T cells in all three patients. Some of the TCR Vβ gene families showed oligoclonal expansions but polyclonal patterns were dominating. The CDR3 distribution of most Vβ families differed between muscle infiltrating lymphocytes and PBL indicating that T cells have expanded locally or selectively accumulated in muscle.
ISSN:0165-5728
1872-8421
DOI:10.1016/S0165-5728(98)00163-5