Immunolocalization of the High-Mobility Group N2 protein and acetylated histone H3K14 in early developing parthenogenetic bovine embryos derived from oocytes of high and low developmental competence

This study investigated differences in the distribution of acetylated histone H3 at Lysine 14 (H3K14ac) and the High‐Mobility Group N2 (HMGN2) protein in the chromatin of early‐ (before 24 hr) and late‐cleaved (after 24 hr) bovine embryos derived from small‐ (1–2 mm) and large‐follicles (4–8 mm). Th...

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Bibliographic Details
Published in:Molecular reproduction and development 2008-02, Vol.75 (2), p.282-290
Main Authors: Bastos, Guilherme M., Gonçalves, Paulo Bayard D., Bordignon, Vilceu
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
bovine
Cattle
embryo development
Embryo, Mammalian - physiology
Embryology: invertebrates and vertebrates. Teratology
Embryonic Development - physiology
Female
follicle size
Fundamental and applied biological sciences. Psychology
General aspects
General aspects. Development. Fetal membranes
H3K14ac
Histones - metabolism
HMGN2
HMGN2 Protein - metabolism
Kinetics
Oocytes - physiology
Organ Culture Techniques
Ovarian Follicle - cytology
Ovarian Follicle - physiology
Parthenogenesis
Zygote - physiology
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Summary:This study investigated differences in the distribution of acetylated histone H3 at Lysine 14 (H3K14ac) and the High‐Mobility Group N2 (HMGN2) protein in the chromatin of early‐ (before 24 hr) and late‐cleaved (after 24 hr) bovine embryos derived from small‐ (1–2 mm) and large‐follicles (4–8 mm). The presence of HMGN2 and H3K14ac has been associated with different nuclear functions including chromatin condensation, transcription, DNA replication and repair. In vitro matured oocytes were parthenogenetically activated (PA) and cultured in synthetic oviduct fluid medium. Early‐ and late‐cleaved embryos were fixed at 36, 50, 60, 70 and 80 hr after PA to detect the presence of H3K14ac and HMGN2. The rates of nuclear maturation (81.1% vs. 58.7%), early cleavage (46.9% vs. 38.9%), and development to blastocyst stage (34.3% vs. 18.9%) were higher (P 
ISSN:1040-452X
1098-2795
DOI:10.1002/mrd.20798