Study of Anti‐Neutrophil Cytoplasmic Antibodies in Patients with Thromboangiitis Obliterans (Buerger’s Disease)

The presence of antineutrophil cytoplasmic antibodies (ANCA) and their specificity to myeloperoxidase, lactoferrin and elastase were studied in 27 patients with thromboangiitis obliterans (Buerger’s disease), including nine in mild stage of the disease (group I) and 18 in active or severe stage (gro...

Full description

Saved in:
Bibliographic Details
Published in:Scandinavian journal of immunology 1998-11, Vol.48 (5), p.544-550
Main Authors: HALACHEVA, K. S, MANOLOVA, I. M, PETKOV, D. P, ANDREEV, A. P.
Format: Article
Language:English
Subjects:
Adult
Antibodies, Antineutrophil Cytoplasmic - blood
Enzyme-Linked Immunosorbent Assay
Female
Fluorescent Antibody Technique, Indirect
Humans
Lactoferrin - immunology
Male
Middle Aged
Neutrophils - immunology
Pancreatic Elastase - immunology
Peroxidase - immunology
Thromboangiitis Obliterans - blood
Thromboangiitis Obliterans - immunology
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The presence of antineutrophil cytoplasmic antibodies (ANCA) and their specificity to myeloperoxidase, lactoferrin and elastase were studied in 27 patients with thromboangiitis obliterans (Buerger’s disease), including nine in mild stage of the disease (group I) and 18 in active or severe stage (group II). Serum samples were investigated for ANCA prevalence by indirect immunofluorescent technique and fixed neutrophil ELISA; the reactivity to myeloperoxidase, lactoferrin and elastase was measured by ELISA. ANCA were found in 55.5% (15/27) of the patients by indirect immunofluorescence and in 59.25% (16/27) by fixed neutrophil ELISA. A highly significant positive association was observed between the values obtained by the two methods. Only six of 15 ANCA‐positive sera reacted with myeloperoxidase and four of 15 with lactoferrin. One ANCA‐negative serum reacted with elastase. Antimyeloperoxidase, antilactoferrin and antielastase autoantibodies were found only in the severe group. There was statistically significant difference in the frequency of ANCA in sera of patients with thromboangiitis obliterans versus control sera. ANCA were predominantly found in group II (66.7%; 12/18) compared to group I (33.3%; 3/9) and the levels of antimyeloperoxidase, antilactoferrin and antielastase antibodies correlated with disease severity. Therefore, detection of ANCA can be a helpful serological test for the diagnosis of thromboangiitis obliterans.
ISSN:0300-9475
1365-3083
DOI:10.1046/j.1365-3083.1998.00430.x