Eosinophil infiltration and degranulation in normal human tissue

Localization of eosinophil granule major basic protein by immunofluorescence permits recognition of both eosinophil infiltration and degranulation. Over the past decade and a half, our laboratory has shown that eosinophil infiltration and degranulation occur in many diseased tissues in humans; among...

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Bibliographic Details
Published in:The Anatomical record 1998-11, Vol.252 (3), p.418-425
Main Authors: Kato, Masahiko, Kephart, Gail M., Talley, Nicholas J., Wagner, Jill M., Sarr, Michael G., Bonno, Motoki, McGovern, Thomas W., Gleich, Gerald J.
Format: Article
Language:English
Subjects:
Adult
Aged
Blood Proteins - analysis
Cell Degranulation - immunology
Cytoplasmic Granules - chemistry
Cytoplasmic Granules - physiology
Eosinophil Granule Proteins
eosinophils
Eosinophils - chemistry
Eosinophils - cytology
Eosinophils - physiology
Female
gastrointestinal tract
Humans
Inflammation Mediators - analysis
Jejunum - cytology
Jejunum - physiology
Lymph Nodes - cytology
Lymph Nodes - physiology
major basic protein
Male
Middle Aged
normal human tissue
Ribonucleases
Thymus Gland - cytology
Thymus Gland - physiology
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Summary:Localization of eosinophil granule major basic protein by immunofluorescence permits recognition of both eosinophil infiltration and degranulation. Over the past decade and a half, our laboratory has shown that eosinophil infiltration and degranulation occur in many diseased tissues in humans; among normal tissues studied as controls, only the gut showed striking eosinophil infiltration and degranulation. Using an indirect immunofluorescence procedure for the detection of major basic protein, we extended our analyses of normal human tissues to include tissues from essentially all body organs; a total of 117 biopsy/ autopsy specimens were analyzed. To determine whether the method of tissue procurement affected the level of eosinophil degranulation in the normal gastrointestinal tract, normal proximal jejunum from six patients was biopsied using either an endoscopic forceps or a scalpel at the time of elective surgery and examined by immunofluorescence. Spleen, lymph node, and thymus tissues showed eosinophil infiltration with scant evidence of degranulation, but the only organ showing both eosinophil infiltration and remarkable degranulation was the gastrointestinal tract. Eosinophil degranulation was significantly increased in specimens obtained by endoscopic forceps compared to those obtained by scalpel (P = 0.021). These results indicate that tissue procurement methods affect the degree of eosinophil degranulation in the gut. Thus, among normal human body organs, both eosinophil infiltration and appreciable degranulation consistently occur only in the gut. Anat. Rec. 252:418–425, 1998. © 1998 Wiley‐Liss, Inc.
ISSN:0003-276X
1097-0185
DOI:10.1002/(SICI)1097-0185(199811)252:3<418::AID-AR10>3.0.CO;2-1