Expression and Imprinting of Insulin-like Growth Factor II (IGF2) and H19 Genes in Uterine Leiomyomas

Genomic imprinting is defined as a gamete of origin-specific epigenetic modification of DNA leading to differential gene expression in the zygote. Several imprinted genes have been identified and some of them are associated with tumor development. We investigated the expression and the imprinting st...

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Bibliographic Details
Published in:Gynecologic oncology 1999-09, Vol.74 (3), p.375-380
Main Authors: Rainho, Cláudia Aparecida, Pontes, Anaglória, Rogatto, Silvia Regina
Format: Article
Language:English
Subjects:
Adult
Biological and medical sciences
Female
Female genital diseases
Gene Expression Regulation, Neoplastic - genetics
Genes, Tumor Suppressor - genetics
genomic imprinting
growth factor
Gynecology. Andrology. Obstetrics
Humans
Insulin-Like Growth Factor II - genetics
Leiomyoma - genetics
Medical sciences
Middle Aged
Muscle Proteins - genetics
RNA, Long Noncoding
RNA, Untranslated
Tumors
Uterine Neoplasms - genetics
uterine tumors
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Summary:Genomic imprinting is defined as a gamete of origin-specific epigenetic modification of DNA leading to differential gene expression in the zygote. Several imprinted genes have been identified and some of them are associated with tumor development. We investigated the expression and the imprinting status of IGF2 and H19 genes in 47 uterine leiomyomas. Using allelic transcription assay, we detected the expression of the IGF2 gene in 10 of a total of 15 informative cases. No loss of imprinting, as determined by the finding of biallelic expression, was detected in any case. The expression of H19 gene was detected in 10 of 20 informative cases and the imprinting pattern was also maintained in all of them. Our data suggest that alterations in IGF2 and H19 genes expression by loss of imprinting do not occur in uterine leiomyomas.
ISSN:0090-8258
1095-6859
DOI:10.1006/gyno.1999.5483