Up-regulation of two Candida albicans genes in the rat model of oral candidiasis detected by differential display

Candida albicans is an opportunistic fungal pathogen responsible for the largest percentage of fungal-mediated oral and oesophageal disease. In this regard, knowledge concerning patterns of gene expression during the establishment and/or maintenance of infection may be the key to the design of new s...

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Bibliographic Details
Published in:Microbial pathogenesis 1998-09, Vol.25 (3), p.121-129
Main Authors: ZHAO, X.-J, NEWSOME, J. T, CIHLAR, R. L
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Biological and medical sciences
Blotting, Southern
Candida albicans
Candida albicans - genetics
Candida albicans - growth & development
Candida albicans - isolation & purification
Candida albicans - pathogenicity
Candidiasis, Oral - microbiology
Disease Models, Animal
DNA, Complementary - analysis
Experimental mycoses and models
False Positive Reactions
Fundamental and applied biological sciences. Psychology
Genes, Fungal
Growth, nutrition, metabolism, transports, enzymes. Molecular biology
Infectious diseases
Medical sciences
Microbiology
Molecular Sequence Data
Mycology
Mycoses
Oropharynx - microbiology
Rats
Reverse Transcriptase Polymerase Chain Reaction
RNA, Fungal - analysis
RNA, Fungal - isolation & purification
RNA, Messenger - analysis
Up-Regulation
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Summary:Candida albicans is an opportunistic fungal pathogen responsible for the largest percentage of fungal-mediated oral and oesophageal disease. In this regard, knowledge concerning patterns of gene expression during the establishment and/or maintenance of infection may be the key to the design of new strategies for treatment, as well as providing insight into pathogenesis. To address this issue, experiments were performed that utilized differential display to compare the spectrum of C. albicans genes expressed during oral infection versus growth in in vitroculture. Experimentally, the rat model of oral candidiasis served as the in vivo source. After initiation of infection and subsequent harvesting of C. albicans from the rat oral cavity, RNA was isolated, and used with a small number of primers in reverse-transcriptase polymerase chain reaction (RT-PCR) and differential display experiments. Fragments unique to in vivo samples were subcloned and sequenced. Southern blot analysis verified the origin of seven fragments as fromC. albicans. Additionally, specific RT-PCR confirmed that two of these fragments represented genes that were up-regulated during C. albicans in vivo growth in the rat model. Database searches indicated the fragments share homology with a member of the C. albicans agglutinin gene family and to a bacterial gene (gidB) possibly involved in cell division.
ISSN:0882-4010
1096-1208
DOI:10.1006/mpat.1998.0218