Characterization of dental follicle cells in developing mouse molar

Dental follicle has been implicated as the origin of alveolar bone, cementum and periodontal ligament, but there is no direct evidence of their cellular lineage. The present pilot study was designed to characterize the phenotype of cultured cells obtained from the dental follicle of neonatal mouse m...

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Bibliographic Details
Published in:Archives of oral biology 1999-09, Vol.44 (9), p.759-770
Main Authors: Hou, Lein-Tuan, Liu, Cheing-Meei, Chen, Yi-Jane, Wong, Man-Ying, Chen, Kun-Chee, Chen, Jinkun, Thomas, Huw F.
Format: Article
Language:English
Subjects:
Alkaline phosphatase
Alkaline Phosphatase - analysis
Alkaline Phosphatase - drug effects
Alkaline Phosphatase - genetics
Animals
Animals, Newborn
Bone sialoprotein
Calcitriol - pharmacology
Calcium Channel Agonists - pharmacology
Cell Lineage
Cells, Cultured
Collagen - analysis
Collagen - genetics
Collagen types
Dental follicle cells
Dental Sac - cytology
Dental Sac - metabolism
Dentistry
Epithelial Cells - cytology
Epithelial Cells - metabolism
Fibronectin
Fibronectins - analysis
Fibronectins - genetics
Gene Expression Regulation
Immunocytochemistry
Immunohistochemistry
In situ hybridization
Integrin-Binding Sialoprotein
Keratins - analysis
Keratins - genetics
Mice
Mice, Inbred Strains
Molar
Osteopontin
Phenotype
Phosphoproteins - analysis
Phosphoproteins - genetics
Pilot Projects
Sialoglycoproteins - analysis
Sialoglycoproteins - genetics
Tooth Germ - cytology
Tooth Germ - metabolism
Tooth Root - cytology
Tooth Root - metabolism
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Summary:Dental follicle has been implicated as the origin of alveolar bone, cementum and periodontal ligament, but there is no direct evidence of their cellular lineage. The present pilot study was designed to characterize the phenotype of cultured cells obtained from the dental follicle of neonatal mouse molars. Developing mandibular molars from 6-day-old CD-1 mice were subjected to 1% trypsin in Hank’s balanced salt solution. After trypsinization, the dental follicle was enucleated from the tooth germ and separated from the associated epithelial root sheath. Pure dental follicle tissue was cultured in α-minimal essential medium containing 10% fetal bovine serum and antibiotics. The nature of the cultured follicle cells was determined in situ by immunocytochemical staining for type I and III collagen, fibronectin, and alkaline phosphatase expression. Earlier phenotypic markers for mineralization such as bone sialoprotein and osteopontin were also examined by in situ hybridization of matched molar tissues. The extracellular matrix proteins (such as type I collagen and fibronectin) were moderately expressed cytochemically. However, type III collagen was strongly stained. Gene expression of bone sialoprotein and osteopontin was detected in sections of mouse molars of similar age. The ALPase activity showed moderate to strong intensity in these primary cultured cells and responded to 1,25(OH) 2 vitamin D 3 treatment. Cytokeratin stains were not noted in these cells. In conclusion, the 6-day-old dental follicle cells exhibit partial characteristics of a mineralized tissue-forming phenotype even though the expression of osteopontin, type I collagen and fibronectin was low at this stage.
ISSN:0003-9969
1879-1506
DOI:10.1016/S0003-9969(99)00033-3