Identification of a novel complex between human kallikrein 2 and protease inhibitor-6 in prostate cancer tissue

Human kallikrein (hK) 2 is an arginine-selective serine protease expressed predominantly in the prostate that has an 80% sequence identity with prostate-specific antigen. Expression of hK2 is elevated in the tumor epithelium compared to benign prostate tissue. We have purified, sequenced, and identi...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 1999-08, Vol.59 (16), p.3927-3930
Main Authors: MIKOLAJCZYK, S. D, MILLAR, L. S, MARKER, K. M, RITTENHOUSE, H. G, WOLFERT, R. L, MARKS, L. S, CHARLESWORTH, M. C, TINDALL, D. J
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biomarkers, Tumor
Cytoplasm - metabolism
Humans
Kallikreins - metabolism
Male
Medical sciences
Nephrology. Urinary tract diseases
Prostatic Neoplasms - metabolism
Prostatic Neoplasms - pathology
Protein Binding
Serine Proteinase Inhibitors - metabolism
Serpins - metabolism
Tissue Kallikreins
Tumors of the urinary system
Urinary tract. Prostate gland
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Summary:Human kallikrein (hK) 2 is an arginine-selective serine protease expressed predominantly in the prostate that has an 80% sequence identity with prostate-specific antigen. Expression of hK2 is elevated in the tumor epithelium compared to benign prostate tissue. We have purified, sequenced, and identified a novel hK2 complex in prostate tissue consisting of hK2 and a serine protease inhibitor known as protease inhibitor-6 (PI-6). This 64-kDa SDS-PAGE stable complex is elevated in the tumor and is approximately 10% of total hK2. No comparable complex of prostate-specific antigen was detected. PI-6, also known as cytoplasmic antiprotease, has been characterized as an intracellular inhibitor of trypsin and chymotrypsin-like proteases, which has high homology to plasminogen activator inhibitor 1 and 2. The physiological role of PI-6 in the prostate and its relationship to hK2 and prostate cancer are under investigation.
ISSN:0008-5472
1538-7445