Identification of Copper-Zinc Superoxide Dismutase Gene from Enteroaggregative Escherichia coli

We describe here the identification of sodC gene from enteroaggregative Escherichia coli (EAggEC). A 294bp gene-specific fragment was amplified from the organism by DNA as well as RT-PCR using primers from bacterial sodC sequences. The metal co-factor present in the protein was confirmed by running...

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Bibliographic Details
Published in:MICROBIOLOGY and IMMUNOLOGY 1999, Vol.43(5), pp.481-484
Main Authors: Kumar, Rakesh, Sidhu, Maninder Kaur, Ganguly, Nirmal Kumar, Chakraborti, Anuradha
Format: Article
Language:English
Subjects:
Bacteriology
Biological and medical sciences
Copper
DNA Primers
EAggEC
Escherichia coli
Escherichia coli - genetics
Escherichia coli - pathogenicity
Escherichia coli Infections - microbiology
Escherichia coli Proteins
Feces - microbiology
Fundamental and applied biological sciences. Psychology
Gastrointestinal Diseases - microbiology
Genes, Bacterial
Genetics
Humans
Metalloproteins - genetics
Microbiology
Pathogenesis
Polymerase Chain Reaction
sodC
sodC gene
Superoxide Dismutase - genetics
Zinc
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Summary:We describe here the identification of sodC gene from enteroaggregative Escherichia coli (EAggEC). A 294bp gene-specific fragment was amplified from the organism by DNA as well as RT-PCR using primers from bacterial sodC sequences. The metal co-factor present in the protein was confirmed by running samples in native gels and inhibiting with 2mM potassium cyanide. However, the nonpathogenic E. coli possesses the gene but does not express it. Thus, the presence of copper-zinc superoxide dismutase encoded by sodC was demonstrated for the first time in EAggEC, which means it could be a novel candidate for a virulence marker.
ISSN:0385-5600
1348-0421
DOI:10.1111/j.1348-0421.1999.tb02431.x