Dietary lutein from marigold extract inhibits mammary tumor development in BALB/c mice

High levels of dietary lutein can inhibit mammary tumor growth in mice. However, the antitumor effect of low levels of dietary lutein on mammary tumors is unavailable. Female BALB/c mice and the WAZ-2T (-SA) mammary tumor cell line were used in two experiments. A preliminary tumor cell dose titratio...

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Bibliographic Details
Published in:The Journal of nutrition 1998-10, Vol.128 (10), p.1650-1656
Main Authors: SOON PARK, J, CHEW, B. P, WONG, T. S
Format: Article
Language:English
Subjects:
Animals
Antioxidants
Biological and medical sciences
Breasts
Carcinogenesis, carcinogens and anticarcinogens
Chromatography, High Pressure Liquid
Diet
Dose-Response Relationship, Drug
Female
Foods and miscellaneous
Lipid Peroxidation - drug effects
Liver - drug effects
Liver - metabolism
Lutein - administration & dosage
Lutein - metabolism
Lutein - therapeutic use
Mammary Neoplasms, Experimental - prevention & control
Medical sciences
Mice
Mice, Inbred BALB C
Organ Size - drug effects
Rodents
Spleen - drug effects
Spleen - metabolism
Tumor Cells, Cultured
Tumors
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Summary:High levels of dietary lutein can inhibit mammary tumor growth in mice. However, the antitumor effect of low levels of dietary lutein on mammary tumors is unavailable. Female BALB/c mice and the WAZ-2T (-SA) mammary tumor cell line were used in two experiments. A preliminary tumor cell dose titration study (Experiment 1) was designed to determine the inoculation dose to produce approximately 65% tumor incidence. Mice (n = 10/dose) were inoculated with 0 to 1 x 10(6) tumor cells in the right inguinal mammary fat pad. A tumor cell load of 2.5 x 10(3) cells/inoculation produced approximately 65% tumor incidence. This dose was used in a subsequent study (Experiment 2) of the efficacy of dietary lutein against mammary tumor development. Mice (n = 20/treatment) were fed a semisynthetic diet containing 0, 0.002, 0.02, 0.2 or 0.4% lutein from marigold extract. After 14 d, all mice were inoculated with 2.5 x 10(3) tumor cells, and tumor growth was measured daily for 70 d at which time blood, liver, spleen and tumors were obtained. Lutein + zeaxanthin uptake increased dose-dependently (P < 0.05) with dietary lutein levels from 0 to 0.02% (spleen) or 0.2% (plasma, liver and tumor). Low levels (0.002 and 0.02%) of dietary lutein lowered (P < 0.05) mammary tumor incidence, tumor growth and lipid peroxidation, and increased tumor latency, whereas higher dietary levels (0.2 or 0.4%) were less effective. Therefore, very low amounts of dietary lutein (0.002%) can efficiently decrease mammary tumor development and growth in mice.
ISSN:0022-3166
1541-6100
DOI:10.1093/jn/128.10.1650