Identification of Neural Circuits by Imaging Coherent Electrical Activity with FRET-Based Dyes

We show that neurons that underlie rhythmic patterns of electrical output may be identified by optical imaging and frequency-domain analysis. Our contrast agent is a two-component dye system in which changes in membrane potential modulate the relative emission between a pair of fluorophores. We demo...

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Bibliographic Details
Published in:Neuron (Cambridge, Mass.) Mass.), 1999-07, Vol.23 (3), p.449-459
Main Authors: Cacciatore, Timothy W., Brodfuehrer, Peter D., Gonzalez, Jesus E., Jiang, Tao, Adams, Stephen R., Tsien, Roger Y., Kristan, William B., Kleinfeld, David
Format: Article
Language:English
Subjects:
Animals
Cell Membrane - physiology
Electrophysiology - methods
Fluorescent Dyes
Ganglia, Invertebrate - cytology
Ganglia, Invertebrate - physiology
Hirudo
Interneurons - cytology
Interneurons - physiology
Leeches
Microscopy, Fluorescence - methods
Motor Neurons - cytology
Motor Neurons - physiology
Movement - physiology
nerve cord
Nervous System - cytology
Neural Pathways
Swimming - physiology
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Summary:We show that neurons that underlie rhythmic patterns of electrical output may be identified by optical imaging and frequency-domain analysis. Our contrast agent is a two-component dye system in which changes in membrane potential modulate the relative emission between a pair of fluorophores. We demonstrate our methods with the circuit responsible for fictive swimming in the isolated leech nerve cord. The output of a motor neuron provides a reference signal for the phase-sensitive detection of changes in fluorescence from individual neurons in a ganglion. We identify known and possibly novel neurons that participate in the swim rhythm and determine their phases within a cycle. A variant of this approach is used to identify the postsynaptic followers of intracellularly stimulated neurons.
ISSN:0896-6273
1097-4199
DOI:10.1016/S0896-6273(00)80799-0