Ribosomal small subunit sequence variation within spores of an arbuscular mycorrhizal fungus, Scutellospora sp

Roots of bluebell (Hyacinthoides nonscripta) were sampled from a woodland in Yorkshire, UK and spores of an arbuscular mycorrhizal fungus Scutellospora sp., were obtained from the surrounding soil. Partial small subunit (SSU) ribosomal RNA sequences were amplified from both roots and spores using ei...

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Bibliographic Details
Published in:Molecular ecology 1999-06, Vol.8 (6), p.915-921
Main Authors: Clapp, J.P, Fitter, A.H, Young, J.P.W
Format: Article
Language:English
Subjects:
18S
arbuscular mycorrhiza
diversity
DNA Primers
DNA, Fungal - chemistry
DNA, Fungal - genetics
DNA, Fungal - isolation & purification
DNA, Ribosomal - chemistry
DNA, Ribosomal - genetics
Endogonales
fungal spores
Fungi - genetics
Fungi - physiology
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genes
genetic variation
Genetic Variation - genetics
Glomales
Hyacinthoides nonscripta
Molecular Sequence Data
mycorrhizal fungi
nucleotide sequences
Phylogeny
Plant Roots - microbiology
Polymerase Chain Reaction - methods
ribosomal DNA
ribosomal RNA
RNA, Ribosomal - genetics
roots
rRNA
Scutellospora
Sequence Analysis, DNA
Spores, Fungal - genetics
SSU
VANS1
vesicular arbuscular mycorrhizae
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Description
Summary:Roots of bluebell (Hyacinthoides nonscripta) were sampled from a woodland in Yorkshire, UK and spores of an arbuscular mycorrhizal fungus Scutellospora sp., were obtained from the surrounding soil. Partial small subunit (SSU) ribosomal RNA sequences were amplified from both roots and spores using either the universal forward primer SS38 or the Glomales‐specific primer VANS1, with the reverse Gigasporaceae‐specific primer VAGIGA. Amplified products were cloned and sequenced. Both spores and roots yielded sequences related to those known from fungi within the Glomales, with up to four distinct SSU sequences obtained from individual spores. The VANS1 primer‐binding site varied considerably in sequence and only a subset of Scutellospora sequences were amplified when the VANS1 primer was used. In addition to glomalean sequences, a number of different sequences, apparently from ascomycetes, were obtained from both root and spore samples.
ISSN:0962-1083
1365-294X
DOI:10.1046/j.1365-294x.1999.00642.x