De novo DNA methylation independent establishment of maternal imprint on X chromosome in mouse oocytes

In female mouse embryos, the paternal X chromosome (Xp) is preferentially inactivated during preimplantation development and trophoblast differentiation. This imprinted X‐chromosome inactivation (XCI) is partly due to an activating imprint on the maternal X chromosome (Xm), which is set during oocyt...

Full description

Saved in:
Bibliographic Details
Published in:Genesis (New York, N.Y. : 2000) N.Y. : 2000), 2008-12, Vol.46 (12), p.768-774
Main Authors: Chiba, Hatsune, Hirasawa, Ryutaro, Kaneda, Masahiro, Amakawa, Yuko, Li, En, Sado, Takashi, Sasaki, Hiroyuki
Format: Article
Language:English
Subjects:
Alleles
Animals
Blastocyst - metabolism
DNA (Cytosine-5-)-Methyltransferases - genetics
DNA methylation
DNA Methylation - genetics
Female
Gene Expression Regulation, Developmental
Genomic Imprinting
Histones - metabolism
Lysine - chemistry
Lysine - metabolism
Male
Mice
mouse
Oocytes - physiology
oogenesis
Transgenes - genetics
Trophoblasts - metabolism
X Chromosome - genetics
X Chromosome - metabolism
X-chromosome inactivation
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In female mouse embryos, the paternal X chromosome (Xp) is preferentially inactivated during preimplantation development and trophoblast differentiation. This imprinted X‐chromosome inactivation (XCI) is partly due to an activating imprint on the maternal X chromosome (Xm), which is set during oocyte growth. However, the nature of this imprint is unknown. DNA methylation is one candidate, and therefore we examined whether disruptions of the two de novo DNA methyltransferases in growing oocytes affect imprinted XCI. We found that accumulation of histone H3 lysine‐27 trimethylation, a hallmark of XCI, occurs normally on the Xp, and not on the Xm, in female blastocysts developed from the mutant oocytes. Furthermore, the allelic expression patterns of X‐linked genes including Xist and Tsix were unchanged in preimplantation embryos and also in the trophoblast. These results show that a maternal disruption of the DNA methyltransferases has no effect on imprinted XCI and argue that de novo DNA methylation is dispensable for Xm imprinting. This underscores the difference between imprinted XCI and autosomal imprinting. genesis 46:768–774, 2008. © 2008 Wiley‐Liss, Inc.
ISSN:1526-954X
1526-968X
DOI:10.1002/dvg.20438