Expression of genes encoding antioxidant enzymes in human and mouse oocytes during the final stages of maturation

The mRNA expression of five enzymes: catalase, Cu-Zn-superoxide dismutase (Cu-Zn-SOD), Mn-superoxide dismutase (Mn-SOD), glutathione peroxidase (GPX), and γ-glutamylcysteine synthetase (GCS) each involved in protection against free radicals was studied in human and mouse oocytes. In the mouse, oocyt...

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Bibliographic Details
Published in:Molecular human reproduction 1999-08, Vol.5 (8), p.720-725
Main Authors: El Mouatassim, S., Guérin, P., Ménézo, Y.
Format: Article
Language:English
Subjects:
Animals
antioxidant enzymes
Antioxidants - metabolism
Base Sequence
Biological and medical sciences
Blastocyst - enzymology
Catalase - genetics
DNA Primers - genetics
Female
free oxygen radicals
Free Radicals - metabolism
Fundamental and applied biological sciences. Psychology
Gene expression
Gene Expression Regulation, Developmental
Gene Expression Regulation, Enzymologic
Glutamate-Cysteine Ligase - genetics
Glutathione Peroxidase - genetics
Humans
In Vitro Techniques
Mice
Molecular and cellular biology
Molecular genetics
oocyte maturation
Oocytes - enzymology
Oocytes - growth & development
Oocytes - metabolism
Oogenesis - genetics
Oogenesis - physiology
preimplantation embryos
Reactive Oxygen Species - metabolism
RNA, Messenger - genetics
RNA, Messenger - metabolism
Superoxide Dismutase - genetics
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Summary:The mRNA expression of five enzymes: catalase, Cu-Zn-superoxide dismutase (Cu-Zn-SOD), Mn-superoxide dismutase (Mn-SOD), glutathione peroxidase (GPX), and γ-glutamylcysteine synthetase (GCS) each involved in protection against free radicals was studied in human and mouse oocytes. In the mouse, oocytes were collected at different stages of maturation in order to determine the storage of these transcripts. For the human, germinal vesicle (GV) oocytes harvested during intracytoplasmic sperm injection (ICSI) procedures and failed fertilized metaphase II (MII) oocytes were analysed. Human and mouse were compared in order to determine whether the differential developmental capacity of mouse and human preimplantation embryos in culture could be explained by the variations in the patterns of expression for these enzymes. mRNA expression for these enzymes was examined using reverse transcription–polymerase chain reaction (RT–PCR). In the mouse, all transcripts (except for catalase) were detected, whatever the maturation stage. No qualitative differences were detected between GV and MII oocytes. In human, all the enzymes (except for catalase) were expressed in MII oocytes and Cu-Zn-SOD was particularly highly expressed. Transcripts corresponding to GPX and Mn-SOD were not detected at GV stage but only at MII stage, suggesting that storage could occur between GV and MII stages. However, using 3′ end-specific primers for GPX and Mn-SOD, instead of the oligo(dT)12–18 primer, for the reverse transcription reaction, the transcripts for these antioxidants enzymes have been detected in human oocytes at the GV stage. This suggests the presence of maturation-specific polyadenylation of these transcripts. These enzymes can be considered as markers of cytoplasmic maturation.
ISSN:1360-9947
1460-2407
1460-2407
DOI:10.1093/molehr/5.8.720