Implication of soluble and membrane HLA class I and serum IL-10 in liver graft acceptance

Membrane HLA class-I expression (mHLA-I), soluble HLA class-I antigens (sHLA-I) and interleukin (IL)-10 are different factors implicated in the special acceptance of liver allograft. In this study, pre- and post-operative levels of mHLA-I in peripheral blood lymphocytes (PBL) and serum sHLA-I were a...

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Published in:Human immunology 1999-06, Vol.60 (6), p.500-509
Main Authors: Minguela, Alfredo, Torı́o, Alberto, Marı́n, Luis, Muro, Manuel, Villar, Luisa M, Dı́az, Julian, Ramı́rez, Pablo, Parrilla, Pascual, Garcı́a-Alonso, Ana M, Álvarez-López, Marı́a R
Format: Article
Language:English
Subjects:
acute rejection
Cell Membrane - immunology
Graft Rejection - immunology
Graft Rejection - therapy
Histocompatibility Antigens Class I - immunology
HLA class I expression
HLA-DR Antigens - immunology
Humans
IL-10
Interleukin-10 - blood
liver transplant tolerance
Liver Transplantation - immunology
Prognosis
Solubility
soluble HLA class I
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Summary:Membrane HLA class-I expression (mHLA-I), soluble HLA class-I antigens (sHLA-I) and interleukin (IL)-10 are different factors implicated in the special acceptance of liver allograft. In this study, pre- and post-operative levels of mHLA-I in peripheral blood lymphocytes (PBL) and serum sHLA-I were analyzed in 86 liver transplants, immunosuppressed with Cyclosporine-A, methylprednisolone and azathioprine, and classified into acute-rejection (AR, n = 28) and non-acute-rejection (NAR, n = 58) groups. Serum IL-10 was studied in 47 recipients (AR-group, n = 16 and NAR-group, n = 31). Pre-transplant values of mHLA-I and sHLA-I showed a bimodal distribution (high/low) in NAR-recipients, but in AR-patients were mainly included in the low expression/secretion zone (mHLA-I, p < 0.02 and sHLA-I, p < 0.05). Consequently, average pre-transplant mHLA-I (868 ± 109 versus 998 ± 123, p < 0.05) and sHLA-I (1.3 ± 0.4 versus 2.02 ± 0.7 μg/ml, p < 0.01) was lower in the AR- than in the NAR-group. After transplant both parameters decreased in the NAR-group, but increased in AR-recipients previous to and on rejection diagnosis day. Additionally, serum IL-10 levels were significantly higher ( p < 0.01) in the NAR than in the AR-group during the first 24 h post-transplant. In conclusion, low pre-transplant mHLA-I and sHLA-I levels pre-dispose liver recipients to acute rejection, whereas early post-transplant increases of serum IL-10 appear to be related to a good liver allograft acceptance.
ISSN:0198-8859
1879-1166
DOI:10.1016/S0198-8859(99)00016-6