Effect of Parenteral Antibiotic Administration on Persistence of Vancomycin-Resistant Enterococcus faecium in the Mouse Gastrointestinal Tract

A mouse model of vancomycin-resistant Enterococcus faecium (VRE) intestinal colonization was used to study the effect of different subcutaneous antibiotics on persistence and density of VRE colonization. Gastric inoculation of a clinical VanB VRE isolate, in conjunction with oral vancomycin in drink...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of infectious diseases 1999-08, Vol.180 (2), p.384-390
Main Authors: Donskey, Curtis J., Hanrahan, Jennifer A., Hutton, Rebecca A., Rice, Louis B.
Format: Article
Language:English
Subjects:
Anaerobic bacteria
Animals
Anti-Bacterial Agents - administration & dosage
Anti-Bacterial Agents - pharmacology
Antibacterial agents
Antibiotics
Antibiotics. Antiinfectious agents. Antiparasitic agents
Antimicrobials
Bacterial diseases
Biological and medical sciences
Cephalosporins
Colony Count, Microbial
Digestive System - microbiology
Drug Resistance, Microbial
Enterococcus faecium
Enterococcus faecium - drug effects
Enterococcus faecium - growth & development
Enterococcus faecium - isolation & purification
Experimental bacterial diseases and models
Feces - microbiology
Female
Flora
Health care administration
Humans
Infections
Infectious diseases
Injections, Subcutaneous
Major Articles
Medical sciences
Mice
Microbial colonization
Pharmacology. Drug treatments
Vancomycin - administration & dosage
Vancomycin - pharmacology
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A mouse model of vancomycin-resistant Enterococcus faecium (VRE) intestinal colonization was used to study the effect of different subcutaneous antibiotics on persistence and density of VRE colonization. Gastric inoculation of a clinical VanB VRE isolate, in conjunction with oral vancomycin in drinking water (250 µg/mL), resulted in high-level VRE colonization (mean, 9.5 log10 cfu/g) in all 169 experimental mice. After discontinuation of oral vancomycin, the level of VRE in the stool specimens of mice receiving subcutaneous saline steadily decreased (mean, 3.59 log10 cfu/g at day 19). Subcutaneous vancomycin, clindamycin, piperacillin-tazobactam, ticarcillin-clavulanic acid, metronidazole, cefotetan, ampicillin, and ampicillin-sulbactam all promoted persistent high levels of stool VRE. Subcutaneous ceftriaxone, cefepime, ciprofloxacin, and aztreonam promoted increased VRE density to a lesser degree or not at all. Thus, in a mouse model, vancomycin and antibiotics with potent antianaerobic activity promoted persistent high-density intestinal VRE colonization, whereas antibiotics lacking potent antianaerobic activity did not.
ISSN:0022-1899
1537-6613
DOI:10.1086/314874