Evaluation of reference genes for studies of gene expression in the bovine liver, kidney, pituitary, and thyroid

Expression patterns of candidate genes with important functions in animal metabolism can help to identify potential molecular markers for cattle production traits. Reverse transcription followed by polymerase chain reaction is a method for rapid and accurate mRNA quantification. However, for exact c...

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Bibliographic Details
Published in:Journal of applied genetics 2008-01, Vol.49 (4), p.367-372
Main Authors: Lisowski, Paweł, Pierzchała, Mariusz, Gościk, Joanna, Pareek, Chandra S., Zwierzchowski, Lech
Format: Article
Language:English
Subjects:
Animal Genetics and Genomics
Animals
Biomarkers - analysis
Biomedical and Life Sciences
Cattle - genetics
Gene Expression
Human Genetics
Kidney - metabolism
Life Sciences
Liver - metabolism
Male
Microbial Genetics and Genomics
Origial Article
Pituitary Gland - metabolism
Plant Genetics and Genomics
Polymerase Chain Reaction
Reference Standards
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - metabolism
Thyroid Gland - metabolism
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Summary:Expression patterns of candidate genes with important functions in animal metabolism can help to identify potential molecular markers for cattle production traits. Reverse transcription followed by polymerase chain reaction is a method for rapid and accurate mRNA quantification. However, for exact comparison of mRNA quantity in various samples or tissues, it is important to choose appropriate reference genes. In cattle, little information is available on the expression stability of housekeeping genes (HKGs). The aim of the present study is to develop a set of reference genes that can be used for normalization of concentrations of mRNAs of genes expressed in the bovine liver, kidney, pituitary and thyroid. The study was performed on 6-, 9-, and 12-month-old bulls of dairy and meat cattle breeds. Six HKGs were investigated: ACTB, GAPDH, HPRTI, SDHA, TBP , and YWHAZ . The most stably expressed potential reference HKGs differed among tissues/organs examined: ACTB, TBP, YWHAZ, GAPDH, HPR TI , and SDHA in the liver; GAPDH and YWHAZ in the kidney; GAPDH and SDHA in the pituitary; and TBP and HPRTI in the thyroid. The results showed that the use of a single gene fornormalization may lead to relatively large errors, so it is important to use multiple control genes based on a survey of potential reference genes applied to representative samples from specific experimental conditions.
ISSN:1234-1983
2190-3883
DOI:10.1007/BF03195635