Effects of VEGF and FGF2 on the Revascularization of Severed Human Dental Pulps

The long-term outcome of replanted avulsed permanent teeth is frequently compromised by lack of revascularization, resulting in pulp necrosis. The purpose of this study was to evaluate the effects of vascular endothelial growth factor (VEGF) and fibroblast growth factor (FGF-2) on the revascularizat...

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Bibliographic Details
Published in:Journal of dental research 2008-12, Vol.87 (12), p.1144-1148
Main Authors: Mullane, E.M., Dong, Z., Sedgley, C.M., Hu, J.C.-C., Botero, T.M., Holland, G.R., Nör, J.E.
Format: Article
Language:English
Subjects:
Adolescent
Adult
Angiogenic Proteins - pharmacology
Animals
Culture Media
Dental Pulp - blood supply
Dental Pulp - drug effects
Dental Pulp - injuries
Dental Pulp - transplantation
Dentistry
Fibroblast Growth Factor 2 - pharmacology
Humans
Mice
Mice, SCID
Microvessels - drug effects
Microvessels - pathology
Neovascularization, Physiologic - drug effects
Subcutaneous Tissue - surgery
Time Factors
Tissue Culture Techniques
Transplants
Vascular Endothelial Growth Factor A - pharmacology
Young Adult
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Summary:The long-term outcome of replanted avulsed permanent teeth is frequently compromised by lack of revascularization, resulting in pulp necrosis. The purpose of this study was to evaluate the effects of vascular endothelial growth factor (VEGF) and fibroblast growth factor (FGF-2) on the revascularization of severed human dental pulps. Tooth slices were prepared from non-carious human molars and treated with 0–50 ng/mL rhVEGF165 or rhFGF-2 for 7 days in vitro. Both angiogenic factors enhanced pulp microvessel density compared with untreated controls (p < 0.05). Tooth slices were also treated with 0 or 50 ng/mL rhVEGF165 for one hour prior to implantation into the subcutaneous space of immunodeficient mice. Treatment with rhVEGF165 increased pulp microvessel density in vivo (p < 0.05). These results demonstrate that rhVEGF165 enhanced neovascularization of severed human dental pulps and suggest that topical application of an angiogenic factor prior to replantation might be beneficial for the treatment of avulsed teeth.
ISSN:0022-0345
1544-0591
DOI:10.1177/154405910808701204