Antioxidant and antimicrobial effects of phenolic compounds extracts of Northeast Portugal honey

Phenolic compounds of dark and clear honeys from Trás-os-Montes of Portugal were extracted with Amberlite XAD-2 and evaluated for their antioxidant and antimicrobial activities. The antioxidant effect was studied using the in vitro test capacity of scavenge the 2,2-diphenyl-1-picryhydrazyl (DPPH) fr...

Full description

Saved in:

Bibliographic Details
Published in:	Food and chemical toxicology 2008-12, Vol.46 (12), p.3774-3779
Main Authors:	Estevinho, Letícia, Pereira, Ana Paula, Moreira, Leandro, Dias, Luís G., Pereira, Ermelinda
Format:	Article
Language:	eng
Subjects:	Anti-Infective Agents antibacterial properties Antimicrobial activity Antioxidant activity Antioxidants Bacillus subtilis Biological and medical sciences Biphenyl Compounds botanical composition Chromatography, High Pressure Liquid Escherichia coli extraction free radical scavengers Free Radical Scavengers - pharmacology Gram-negative bacteria Gram-Negative Bacteria - drug effects Gram-positive bacteria Gram-Positive Bacteria - drug effects Honey Honey - analysis honey and bee products Indicators and Reagents Klebsiella pneumoniae Medical sciences Microbial Sensitivity Tests Oxidation-Reduction Phenolic compounds Phenols - isolation & purification Phenols - pharmacology Picrates - chemistry Portugal Pseudomonas aeruginosa Reference Standards Staphylococcus aureus Staphylococcus lentus Streptococcus pneumoniae Toxicology
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Phenolic compounds of dark and clear honeys from Trás-os-Montes of Portugal were extracted with Amberlite XAD-2 and evaluated for their antioxidant and antimicrobial activities. The antioxidant effect was studied using the in vitro test capacity of scavenge the 2,2-diphenyl-1-picryhydrazyl (DPPH) free radical and of reducing power of iron (III)/ferricyanide complex. The antimicrobial activity was screened using three Gram-positive bacteria ( Bacillus subtilis, Staphylococcus aureus, Staphylococcus lentus) and three Gram-negative bacteria ( Pseudomonas aeruginosa, Klebsiella pneumoniae and Escherichia coli). The results obtained from the partial identification of honey phenolic compounds by high-performance liquid chromatography with a diode array detector showed that p-hydroxibenzoic acid, cinnamic acid, naringenin, pinocembrin and chrysin are the phenolic compounds present in most of the samples analyzed. Antioxidant potential was dependent of honey extract concentration and the results showed that dark honey phenolic compounds had higher activity than the obtained from clear honey. In the biological assays, results showed that S. aureus were the most sensitive microrganisms and B. subtilis, S. lentus, K. pneumoniae and E. coli were each moderately sensitive to the antimicrobial activity of honey extracts. Nevertheless, no antimicrobial activity was observed in the test with P. aeruginosa.
ISSN:	0278-6915 1873-6351