The origin of marginal zone B cells in the rat

The marginal zone is a unique compartment that is only found in the spleen. Rat marginal zone B cells (MZ‐B) can be distinguished from other B cells, e.  g. recirculating follicular B cells (RF‐B), by several phenotypic characteristics. Typically MZ‐B cells are surface (s)IgMhi, sIgDlo and CD45R(B22...

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Bibliographic Details
Published in:European journal of immunology 1999-05, Vol.29 (5), p.1522-1531
Main Authors: Dammers, Peter M., de Boer, Nynke K., Deenen, Gerrit Jan, Nieuwenhuis, Paul, Kroese, Frans G. M.
Format: Article
Language:English
Subjects:
Adriamycin
Animals
B cell
B-Lymphocyte Subsets - immunology
B-Lymphocytes - classification
B-Lymphocytes - cytology
B-Lymphocytes - immunology
Cell Differentiation
Differentiation
Doxorubicin
Flow Cytometry
Lymphocytes
Male
Marginal zone
Mice
NF-kappa B - metabolism
Rat
Rats
Spleen - cytology
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Summary:The marginal zone is a unique compartment that is only found in the spleen. Rat marginal zone B cells (MZ‐B) can be distinguished from other B cells, e.  g. recirculating follicular B cells (RF‐B), by several phenotypic characteristics. Typically MZ‐B cells are surface (s)IgMhi, sIgDlo and CD45R(B220)lo, whereas RF‐B cells are sIgMlo, sIgDhi and CD45Rhi. In addition, MZ‐B cells stain strongly with HIS57, a newly developed monoclonal antibody. The developmental pathway and origin of MZ‐B cells are not exactly known. However, previous studies indicate that recirculating (i.  e. thoracic duct) B cells can give rise to MZ‐B cells. Here the origin of (naive) MZ‐B cells was studied using adriamycin (doxorubicin)‐induced B cell depletion. Using three‐color flow cytometry and immunohistology we show that 2 days after a single i.  v. injection of the anti‐tumor drug adriamycin only RF‐B cells can be detected, while all other B cell subpopulations are depleted, including all bone marrow precursor B cells. By studying the sequential reappearance of various B cell subsets and their precursors after adriamycin administration we show that MZ‐B cells and the splenic marginal zone can be detected at a time point at which newly generated B cells (immature B cells) are not yet present. Given the observation that only RF‐B cells were present at this time, we conclude that RF‐B cells are the immediate MZ‐B precursor cells.
ISSN:0014-2980
1521-4141
DOI:10.1002/(SICI)1521-4141(199905)29:05<1522::AID-IMMU1522>3.0.CO;2-0