Studies on the production of conjugated linoleic acid from linoleic and vaccenic acids by mixed rumen protozoa

The present study was designed to investigate the capability of mixed rumen protozoa to synthesize conjugated linoleic acid (CLA) from linoleic (LA) and vaccenic acids (VA). Rumen contents were collected from fistulated cows. The protozoal fraction was separated and washed several times with MB9 buf...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2008-12, Vol.81 (3), p.533-541
Main Authors: Or-Rashid, Mamun M, AlZahal, Ousama, McBride, Brian W
Format: Article
Language:English
Subjects:
Acids
Animals
Applied Microbial and Cell Physiology
Bacteria
Biological and medical sciences
Biotechnology
Cattle
CLA
Eukaryota - chemistry
Eukaryota - isolation & purification
Eukaryota - metabolism
Female
Fundamental and applied biological sciences. Psychology
Hydrogenation
Isomerism
Life Sciences
linoleic acid
Linoleic Acid - chemistry
Linoleic Acid - metabolism
Linoleic Acids, Conjugated - chemistry
Linoleic Acids, Conjugated - metabolism
Microbial Genetics and Genomics
Microbiology
Oleic Acids - chemistry
Oleic Acids - metabolism
Protozoa
Rumen - metabolism
Rumen - parasitology
rumen protozoa
Studies
vaccenic acid
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Summary:The present study was designed to investigate the capability of mixed rumen protozoa to synthesize conjugated linoleic acid (CLA) from linoleic (LA) and vaccenic acids (VA). Rumen contents were collected from fistulated cows. The protozoal fraction was separated and washed several times with MB9 buffer and then resuspended in autoclaved rumen fluid. The suspensions were anaerobically incubated up to 18 h at 38.5 °C with substrates in the presence (P-AB) or the absence of antibacterial-agents (P-No-AB). Neither P-AB nor P-No-AB suspensions were capable of producing CLA from VA (11t-18:1). Linoleic acid was catabolized by P-No-AB to a greater extent than P-AB. Different isomers of CLA were synthesized by P-AB from LA. The 9c11t-CLA was predominant. Thirty seven percent of the maximum accumulated 9c11t-CLA was found in the P-AB suspension as early as 0.1 h into the incubation period. Accumulation of 10t12c-CLA in P-AB suspension was approximately 10.0 times lower than that of 9c11t-CLA. There were no significant productions of VA, 10t-18:1, and 18:0 in P-AB compared with the control, indicating that rumen protozoa have no ability to biohydrogenate CLA isomers. On the other hand, the concentrations of 10t-18:1, VA, and 18:0 in P-No-AB were greater (P < 0.05) compared with those in P-AB, indicating the role of symbiotic bacteria associated with P-No-AB in biohydrogenating CLA isomers. We concluded that mixed rumen protozoa are capable of synthesizing CLA from LA through isomerization reactions. However, they are incapable of metabolizing CLA further. They are also incapable of vaccenic acid biohydrogenation and/or desaturation.
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-008-1690-0