Immunohistochemical analysis of lens cells on formation of different types of age-related cataract in humans

The aim of the present work was to perform a comparative immunohistochemical study of the ocular lens in normal conditions and after formation of various type of age-related cataract in humans. The control group consisted of lenses from young men dying from accidents and serving as corneal donors (...

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Bibliographic Details
Published in:Neuroscience and behavioral physiology 2008-11, Vol.38 (9), p.887-890
Main Authors: Korsakova, N. V., Sergeeva, V. E., Petrov, S. B.
Format: Article
Language:English
Subjects:
Actins - metabolism
Adult
Age
Aged
Aging
Behavioral Sciences
Biomedical and Life Sciences
Biomedicine
Cataract - metabolism
Cataract - pathology
Cataracts
Genotype & phenotype
Humans
Hydration
Immunohistochemistry
Keratins - metabolism
Lens Cortex, Crystalline - metabolism
Lens Cortex, Crystalline - pathology
Lens Nucleus, Crystalline - metabolism
Lens Nucleus, Crystalline - pathology
Male
Middle Aged
Monoclonal antibodies
Morphology
Neurobiology
Neurosciences
Phosphopyruvate Hydratase - metabolism
S100 Proteins - metabolism
Surgery
Vimentin - metabolism
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Summary:The aim of the present work was to perform a comparative immunohistochemical study of the ocular lens in normal conditions and after formation of various type of age-related cataract in humans. The control group consisted of lenses from young men dying from accidents and serving as corneal donors ( n = 10, not more than one hour after death). Lenses from patients (aged 60–70 years) undergoing surgery for cortical ( n = 20) and nuclear ( n = 20) cataracts were studied. The investigations demonstrated plasticity in the phenotype of lens cells in age-related cataract. Changes in the phenotype of lens cells were found to depend on the type of age-related cataract. Development of the cortical variant of age-related cataract was associated with positive reactions with monoclonal antibodies to neuron-specific enolase, S-100 protein, and vimentin only within the lens cortex. Formation of age-related nuclear cataract was associated with positive reactions with antibodies to α-smooth muscle actin and pancytokeratin, which were detected only in the lens nucleus. This provides evidence of the need to develop a differential approach to the treatment of different types of age-related cataract.
ISSN:0097-0549
1573-899X
DOI:10.1007/s11055-008-9066-6