AtrbohD-Mediated Oxidative Burst Elicited by Oligogalacturonides in Arabidopsis Is Dispensable for the Activation of Defense Responses Effective against Botrytis cinerea

Oligogalacturonides (OGs) are endogenous elicitors of defense responses released after partial degradation of pectin in the plant cell wall. We have previously shown that, in Arabidopsis (Arabidopsis thaliana), OGs induce the expression of PHYTOALEXIN DEFICIENT3 (PAD3) and increase resistance to the...

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Published in:Plant physiology (Bethesda) 2008-11, Vol.148 (3), p.1695-1706
Main Authors: Galletti, Roberta, Denoux, Carine, Gambetta, Stefano, Dewdney, Julia, Ausubel, Frederick M, De Lorenzo, Giulia, Ferrari, Simone
Format: Article
Language:English
Subjects:
Arabidopsis - enzymology
Arabidopsis - metabolism
Arabidopsis - microbiology
Biological and medical sciences
Botrytis
Botrytis - pathogenicity
Cyclopentanes - metabolism
Ethylenes - metabolism
Fundamental and applied biological sciences. Psychology
Fungal plant pathogens
Genes
Hexuronic Acids - metabolism
Infections
Leaves
Mitochondrial Proteins
Oxidases
Oxidoreductases - metabolism
Oxylipins - metabolism
Pathogens
Phytopathology. Animal pests. Plant and forest protection
Plant cells
Plant physiology and development
Plant Proteins
Plants
Plants Interacting with Other Organisms
Reactive oxygen species
Respiratory Burst
Salicylic Acid - metabolism
Seedlings
Signal Transduction
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Summary:Oligogalacturonides (OGs) are endogenous elicitors of defense responses released after partial degradation of pectin in the plant cell wall. We have previously shown that, in Arabidopsis (Arabidopsis thaliana), OGs induce the expression of PHYTOALEXIN DEFICIENT3 (PAD3) and increase resistance to the necrotrophic fungal pathogen Botrytis cinerea independently of signaling pathways mediated by jasmonate, salicylic acid, and ethylene. Here, we illustrate that the rapid induction of the expression of a variety of genes by OGs is also independent of salicylic acid, ethylene, and jasmonate. OGs elicit a robust extracellular oxidative burst that is generated by the NADPH oxidase AtrbohD. This burst is not required for the expression of OG-responsive genes or for OG-induced resistance to B. cinerea, whereas callose accumulation requires a functional AtrbohD. OG-induced resistance to B. cinerea is also unaffected in powdery mildew resistant4, despite the fact that callose accumulation was almost abolished in this mutant. These results indicate that the OG-induced oxidative burst is not required for the activation of defense responses effective against B. cinerea, leaving open the question of the role of reactive oxygen species in elicitor-mediated defense.
ISSN:0032-0889
1532-2548
1532-2548
DOI:10.1104/pp.108.127845