Evidence that the neuronal nitric oxide synthase inhibitor 7-nitroindazole inhibits monoamine oxidase in the rat: in vivo effects on extracellular striatal dopamine and 3,4-dihydroxyphenylacetic acid

The present study investigated in vivo the kinetic of the changes in rat striatal extracellular concentrations of dopamine (DA), and its monoamine oxidase (MAO)-derived metabolite 3,4-dihydroxyphenylacetic acid (DOPAC), following administration either of nitric oxide (NO) synthase (NOS) inhibitors 7...

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Bibliographic Details
Published in:Neuroscience letters 1999-04, Vol.264 (1), p.5-8
Main Authors: Desvignes, Christophe, Bert, Lionel, Vinet, Laurent, Denoroy, Luc, Renaud, Bernard, Lambás-Señas, Laura
Format: Article
Language:English
Subjects:
3,4-dihydroxyphenylacetic acid
3,4-Dihydroxyphenylacetic Acid - metabolism
7-nitroindazole
Animals
Biochemistry and metabolism
Biological and medical sciences
Central nervous system
Differential normal pulse voltammetry
Dopamine
Dopamine - metabolism
Electrophysiology - methods
Enzyme Inhibitors - pharmacology
Extracellular Space - metabolism
Fundamental and applied biological sciences. Psychology
Indazoles - pharmacology
Male
Microdialysis
Monoamine oxidase
Monoamine Oxidase Inhibitors - pharmacology
NG-Nitroarginine Methyl Ester - pharmacology
Nitric oxide synthase
Nitric Oxide Synthase - antagonists & inhibitors
Nitric Oxide Synthase Type I
Pargyline - pharmacology
Rats
Rats, Sprague-Dawley
Striatum
Vertebrates: nervous system and sense organs
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Summary:The present study investigated in vivo the kinetic of the changes in rat striatal extracellular concentrations of dopamine (DA), and its monoamine oxidase (MAO)-derived metabolite 3,4-dihydroxyphenylacetic acid (DOPAC), following administration either of nitric oxide (NO) synthase (NOS) inhibitors 7-nitroindazole (7-NI) and N ω -nitro- l-arginine methyl ester (L-NAME) or of the widely used MAO inhibitor pargyline. DA and DOPAC concentrations were determined every 4 min by microdialysis combined with capillary zone electrophoresis coupled with laser-induced fluorescence detection (CZE-LIFD) and by differential normal pulse voltammetry (DNPV), respectively. Administration of 7-NI, both systemic (30 mg/kg, intraperitoneally, i.p.) or intrastriatal (1 mM through the microdialysis probe), as well as administration of pargyline (75 mg/kg, i.p.), induced simultaneously in the striatum a significant increase in extracellular DA and a significant decrease in extracellular DOPAC. However, administration of L-NAME (200 mg/kg, i.p.) produced a significant increase in striatal extracellular DA without changes in extracellular DOPAC. These data suggest a possible MAO inhibitory effect of 7-NI which seems to be restricted to this NOS inhibitor. These results may be of special interest for the studies on functional role of NO in the brain, particularly in dopaminergic transmission.
ISSN:0304-3940
1872-7972
DOI:10.1016/S0304-3940(99)00139-1