Gender preselection in cattle with intracytoplasmically injected, flow cytometrically sorted sperm heads

We investigated the development to the blastocyst and subsequent live-offspring stages of in vitro-matured bovine oocytes intracytoplasmically injected with flow cytometrically sorted bull sperm heads. Bull sperm heads, prepared by ultrasound sonication, were distinguished and sorted on the basis of...

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Bibliographic Details
Published in:Biology of reproduction 1999-05, Vol.60 (5), p.1194-1197
Main Authors: Hamano, K, Li, X, Qian, X.Q, Funauchi, K, Furudate, M, Minato, Y
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cattle
Cell Separation - methods
DNA - chemistry
embryo culture
Embryo Transfer
embryogenesis
Female
Fertilization in Vitro
Flow Cytometry
Fundamental and applied biological sciences. Psychology
In Vitro Techniques
injection
intracytoplasmic sperm injectin
Male
Mammalian male genital system
Morphology. Physiology
oocytes
Oocytes - growth & development
Pregnancy
sex control
sex determination
sex preselection
Sex Preselection - methods
sorting
Sperm Head - physiology
sperm sorting
spermatozoa
Vertebrates: reproduction
X Chromosome
Y Chromosome
y sperm
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Summary:We investigated the development to the blastocyst and subsequent live-offspring stages of in vitro-matured bovine oocytes intracytoplasmically injected with flow cytometrically sorted bull sperm heads. Bull sperm heads, prepared by ultrasound sonication, were distinguished and sorted on the basis of their relative DNA contents using a flow cytometer/cell sorter modified for sorting sperm. By fluorescence in situ hybridization, the proportion of sperm confirmed as having Y specific DNA in the fraction sorted for the Y sperm was 82%. Injection with single sorted sperm heads of in vitro-matured oocytes (cultured for 24 h) resulted in 46.6% cleavage and 6.9% blastocyst development rates. Embryo transfer of 48 blastocysts (Days 7–8) to recipients (one per recipient) resulted in 20.8% pregnancy and 20.8% normal live offspring production rates. The birth of 8 male and 2 female calves represents an 80% sex preselection accuracy rate.
ISSN:0006-3363
1529-7268
DOI:10.1095/biolreprod60.5.1194